APC

APC

  • Western blot analysis with Mouse Anti-GFP Monoclonal Antibody, Clone H24 (STMA00231) showing detection of recombinant Aequorea GFP protein, his-tagged (Invitrogen A42613). Block: 2% skim milk + 2% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Jellyfish GFP Recombinant Monoclonal [H24] (STMA00231) at 1:5000 for 1 hour at RT. Secondary Antibody: Goat anti-mouse IgG-HRP (H&L) at 1:5000 for 30 minutes at RT. Immunocytochemistry/Immunofluorescence analysis with Mouse Anti-GFP Monoclonal Antibody, Clone H24 (STMA00231). Tissue: Embryonic kidney cells (HEK-293). Species: Human. Transfection: GFP expressing plasmid, incubated for 48 hours. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 1 hour at RT.

    Anti-GFP Antibody [H24]

    GFP Antibody [H24]GFP Antibody [H24] is a mouse recombinant monoclonal antibody directed against GFP, offered by Assay Genie for research applications. Reported applications include WB and ICC/IF. Reactivity is species independent. Supplied as IgG1,...
  • Western blot analysis with Rabbit Anti-Kv1.3 Monoclonal Antibody, Clone CAR1.1 (STMA00234) showing detection of Human Kv1.3 protein in a transient over-expression (OE) lysate (OriGene LY4194581). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat Kv1.3 Recombinant Monoclonal [CAR1.1] (STMA00234) at 1:10,000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis with Rabbit Anti-Kv1.3 Monoclonal Antibody, Clone CAR1.1 (STMA00234). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Rat Kv1.3 Recombinant Monoclonal [CAR1.1] (STMA00234) at 1:100 for 1 hour at RT.

    Anti-Kv1.3 Antibody [CAR1.1]

    Kv1.3 Antibody [CAR1.1]Kv1.3 Antibody [CAR1.1] is a rabbit recombinant monoclonal antibody directed against Kv1.3, offered by Assay Genie for research applications. Reported applications include WB, IHC and ICC/IF. Reported reactivity: Human, Mouse and...
  • Dot blot analysis comparing binding affinity of STMA00226 on 10, 50, and 100 ng of Human Recombinant Alpha Synuclein Monomers, Pre-Formed Fibrils, and Dopamine-Stabilized Oligomers. Block: 5% skim milk for 1 hour at RT. Primary Antibody: Mouse Anti-Human Alpha Synuclein Oligomer [9D2] (STMA00226) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-mouse IgG:HRP (Rockland Cat # 710-1331) at 1:4000 for 1 hour at RT. Sandwich ELISA analysis comparing detection of Human Recombinant Alpha Synuclein Monomers. The range tested for each construct was 0 to 10 ng/mL. Block: 1% BSA + 5% Sucrose in PBS pH 7.4 for 1 hour at RT. Detection antibody: HRP-conjugated and incubated for 1 hour at RT. Colour Development: TMB Substrate.

    Anti-Alpha Synuclein Oligomer Antibody [9D2]

    Alpha Synuclein Oligomer Antibody [9D2]Alpha Synuclein Oligomer Antibody [9D2] is a mouse monoclonal antibody directed against Alpha Synuclein Oligomer, offered by Assay Genie for research applications. Reported applications include WB, IHC and ELISA...
  • Western blot analysis with Rabbit Anti-CASPR2 Monoclonal Antibody, Clone FF19 (STMA00227) showing detection of Human CASPR2 protein in a transient over-expression (OE) lysate (OriGene LY402278). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Human CASPR2 Recombinant Monoclonal [FF19] (STMA00227) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis with Rabbit Anti-CASPR2 Monoclonal Antibody, Clone FF19 (STMA00227). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Human CASPR2 Recombinant Monoclonal [FF19] (STMA00227) at 1:100 for 1 hour at RT.

    Anti-CASPR2 Antibody [FF19]

    CASPR2 Antibody [FF19]CASPR2 Antibody [FF19] is a rabbit recombinant monoclonal antibody directed against CASPR2, offered by Assay Genie for research applications. Reported applications include WB, IHC and ICC/IF. Reported reactivity: Human, Mouse and...
  • Western blot analysis comparing STMA00225 with STPA00086 showing differences in detection of Recombinant Human Alpha Synuclein aa 1-114 Monomer, Recombinant Human Alpha Synuclein-98 Monomer, and Recombinant Human Alpha Synuclein Full-length Monomers. Block: 5% skim milk for 1/2 hour at RT. Immunohistochemistry analysis with Mouse Anti-Human Alpha Synuclein N-Terminal Monoclonal Antibody, Clone 11D4 (STMA00225). Tissue: Striatum sections of Parkinson’s Diseased Brain. Species: Human. Fixation: Paraffin embedded, 5um thin. Blocking: 3% milk for 1 hour at RT. Antigen Retrieval: Citrate buffer, pH 6.0, boiled for 20 min. Primary Antibody: Mouse Anti-Human Alpha Synuclein N-Terminal Monoclonal Antibody, Clone 11D4 (STMA00225).

    Anti-Alpha Synuclein N-terminal Antibody [11D4]

    Alpha Synuclein N-terminal Antibody [11D4]Alpha Synuclein N-terminal Antibody [11D4] is a mouse monoclonal antibody directed against Alpha Synuclein N-terminal, offered by Assay Genie for research applications. Reported applications include WB and IHC...
  • Western blot analysis comparing detection of Amyloid Beta 1-42 constructs (amyloid beta 1-42 peptide; amyloid beta 1-42 oligomers; amyloid beta 1-42 pre-formed fibrils; amyloid beta pyroglutamate 3-42 pre-formed fibrils). Block: 5% skim milk. Primary Antibodies: Rabbit Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (STMA00223, clone 1C1) & Mouse Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10), at 1:10,000 & 1:1000. Sandwich ELISA analysis comparing detection of Amyloid Beta constructs. The range tested for each construct was 0 to 2000 pg/mL, and the standard curve was prepared using amyloid beta 1-42 oligomers. Within this range, no cross-reactivity with monomeric peptide & APP 751 was observed.

    Anti-Amyloid Beta 1-42 Oligomer Antibody [1C1]

    Amyloid Beta 1-42 Oligomer Antibody [1C1]Amyloid Beta 1-42 Oligomer Antibody [1C1] is a rabbit recombinant monoclonal antibody directed against Amyloid Beta 1-42 Oligomer, offered by Assay Genie for research applications. Reported applications include WB...
  • Western blot analysis comparing detection of Amyloid Beta 1-42 constructs (amyloid beta 1-42 peptide; amyloid beta 1-42 oligomers; amyloid beta 1-42 pre-formed fibrils; amyloid beta pyroglutamate 3-42 pre-formed fibrils). Block: 5% skim milk. Primary Antibodies: Rabbit Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (STMA00224, clone 2F9) & Mouse Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10), at 1:10,000 & 1:1000. Sandwich ELISA analysis comparing detection of Amyloid Beta constructs. The range tested for each construct was 0 to 5000 pg/mL, and the standard curve was prepared using amyloid beta 1-42 oligomers. Within this range, no cross-reactivity with monomeric peptide & APP 751 was observed.

    Anti-Amyloid Beta 1-42 Oligomer Antibody [2F9]

    Amyloid Beta 1-42 Oligomer Antibody [2F9]Amyloid Beta 1-42 Oligomer Antibody [2F9] is a rabbit recombinant monoclonal antibody directed against Amyloid Beta 1-42 Oligomer, offered by Assay Genie for research applications. Reported applications include WB...
  • Immunohistochemistry (IHC) analysis on a temporal lobe section of a human Alzheimer’s diseased brain tissue. Block: 3% skim milk in PBST. Primary Antibody: Rabbit Anti-Amyloid Beta Oligomer Monoclonal Antibody, clone 1A2 (STMA00222) at 1:100. Secondary: Goat-anti-rabbit IgG:HRP at 1:100. Stained with DAB (1:50) and Hematoxylin (1:10). Western blot analysis comparing detection of Amyloid Beta 1-42 constructs (amyloid beta 1-42 peptide; amyloid beta 1-42 oligomers; amyloid beta 1-42 pre-formed fibrils; amyloid beta pyroglutamate 3-42 pre-formed fibrils). Block: 5% skim milk. Primary Antibodies: Rabbit Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (STMA00222, clone 1A2) & Mouse Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10).

    Anti-Amyloid Beta 1-42 Oligomer Antibody [1A2]

    Amyloid Beta 1-42 Oligomer Antibody [1A2]Amyloid Beta 1-42 Oligomer Antibody [1A2] is a rabbit recombinant monoclonal antibody directed against Amyloid Beta 1-42 Oligomer, offered by Assay Genie for research applications. Reported applications include WB...
  • Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PPM1H Monoclonal Antibody, Clone 10D2 (STMA00221). Tissue: Alveolar Basal Epithelial cells (A549). Species: Hu, Ms, Rt. Primary Antibody: Mouse Anti-PPM1H Monoclonal Antibody (STMA00221) at 1:1000 for 1 hour at RT. Secondary Antibody: Alexa Fluor 594 Donkey anti mouse secondary diluted in 0.2% BSA + 0.02% sodium azide in PBS at 1:1000 for 1 hour at RT. Western Blot analysis of Hu, Ms, Rt Kidney Cells (HEK293), Alveolar Basal Epithelial cells (A549) showing detection of ~55 PPM1H protein using Mouse Anti-PPM1H Monoclonal Antibody, Clone 10D2 (STMA00221). Lane 1: MW Ladder. Lane 2: A549. Lane 3: HEK293. Load: 30 ug. Block: 5% Skim Milk in TBST, 1h, RT. Primary Antibody: Mouse Anti-PPM1H Monoclonal Antibody (STMA00221) at 1:1000 for Overnight, 4C.

    Anti-PPM1H Antibody [10D2]

    PPM1H Antibody [10D2]PPM1H Antibody [10D2] is a mouse monoclonal antibody directed against PPM1H, offered by Assay Genie for research applications. Reported applications include WB and ICC/IF. Reported reactivity: Human, Mouse and Rat. Supplied as IgG1,...
  • Dot Blot analysis of Human, Mouse, Rat Tau Peptide (pThr217) showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 15B7 (STMA00220). Panel A: hTau 2N4R monomer (pSer214) (2 ug). Panel B: Tau peptide (pThr217) (2 ug). Panel C: Tau peptide (non phospho) (2 ug). Load: 2ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (STMA00220) at 1:1000 for 1 hour at RT with shaking. Dot Blot analysis of Human, Mouse, Rat Tau 2N4R P301S (phospho & non-phospho) showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 15B7 (STMA00220). Lane 1: hTau 2N4R P301S Monomer(0.5ug). Lane 2: hTau 2N4R P301S Monomer(1.0ug). Lane 3: Recombinant hTau-441 (2N4R) P301S Monomer, Baculovirus/Sf9 Expressed (phosphorylated)(0.5ug).

    Anti-Tau Antibody (pThr217) [15B7]

    Tau Antibody (pThr217) [15B7]Tau Antibody (pThr217) [15B7] is a mouse monoclonal antibody directed against Tau Antibody (pThr217), offered by Assay Genie for research applications. Reported applications include WB and DB. Reported reactivity: Human,...
  • Western Blot analysis of Human HEK293 cells overexpressing RAB3A, RAB3B, RAB3C, and RAB3D showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9 (STMA00219). Lane 1: MW ladder. Lanes 2-4: HEK293 cells overexpressing RAB3A. Lanes 5-7: HEK293 cells overexpressing RAB3B. Lanes 8-10: HEK293 cells overexpressing RAB3C. Lanes 11-13: HEK293 cells overexpressing RAB3D. Western Blot analysis of Human, Mouse, Rat A549, T-47D cells, mouse and rat brain, kidney showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9 (STMA00219). Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3: T-47D cells. Lane 4: mouse brain. Lane 5: rat brain. Lane 6: mouse kidney. Lane 7: rat kidney. Load: 15 ug. Block: 5% Skim Milk Powder in TBST.

    Anti-RAB3D Antibody [5E9]

    RAB3D Antibody [5E9]RAB3D Antibody [5E9] is a mouse monoclonal antibody directed against RAB3D, offered by Assay Genie for research applications. Reported applications include WB. Reported reactivity: Human, Mouse and Rat. Supplied as IgG1, purified by...
  • Western Blot analysis of Human, Mouse A549 WT and KO cells, MEF, mouse brain cells showing detection of RAB1A protein using Mouse Anti-RAB1A Monoclonal Antibody, Clone 4G10 (STMA00218). Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3-5: KO A549 clones. Lane 6: MEF cells. Lane 7: mouse brain cells. Load: 10 ug (A549, MEF); 20 ug (Mouse Brain). Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody (STMA00218) at 1:1000. Immunoprecipitation analysis using Mouse Anti-RAB1A Monoclonal Antibody, Clone 4G10 (STMA00218). Tissue: HEK293 cells overexpressing RAB1A, RAB1B, and RAB1C. Species: Human. Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody (STMA00218).

    Anti-RAB1A Antibody [4G10]

    RAB1A Antibody [4G10]RAB1A Antibody [4G10] is a mouse monoclonal antibody directed against RAB1A, offered by Assay Genie for research applications. Reported applications include WB and IP. Reported reactivity: Human and Mouse. Supplied as IgG1, purified...