Developmental Biology

Anti-ZP4 Antibody (CAB15180)

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SKU:
CAB15180
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-ZP4 Antibody
Antibody SKU:CAB15180
Antibody Size:20uL, 50uL, 100uL
Application:IHC
Reactivity:Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400-500 of human ZP4 (NP_067009.1).
Application:IHC
Recommended Dilution:IHC 1:50 - 1:200
Reactivity:Rat
Positive Samples:
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400-500 of human ZP4 (NP_067009.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IPVQ KALD LPFP SHHQ RFSI FTFS FVNP TVEK QALR GPVH LHCS VSVC QPAE TPSC VVTC PDLS RRRN FDNS SQNT TASV SSKG PMIL LQAT KDPP EKLR V
Gene ID:57829
Uniprot:Q12836
Cellular Location:Cell membrane, Secreted, Single-pass type I membrane protein, extracellular matrix, extracellular space
Calculated MW:59kDa
Observed MW:Refer to figures
Synonyms:ZP4, ZBP, ZP1, ZPB, Zp-4
Background:The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4.
UniProt Protein Function:ZP4: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor. Belongs to the ZP domain family. ZPB subfamily.
UniProt Protein Details:

Protein type:Extracellular matrix; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: extracellular region

Molecular Function:acrosin binding; signal transducer activity

Biological Process: binding of sperm to zona pellucida; positive regulation of humoral immune response; positive regulation of protein kinase activity; positive regulation of T cell proliferation

NCBI Summary:The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. [provided by RefSeq, Jul 2008]
UniProt Code:Q12836
NCBI GenInfo Identifier:46397080
NCBI Gene ID:57829
NCBI Accession:Q12836.1
UniProt Secondary Accession:Q12836,B2RAE1,
UniProt Related Accession:Q12836
Molecular Weight:59,400 Da
NCBI Full Name:Zona pellucida sperm-binding protein 4
NCBI Synonym Full Names:zona pellucida glycoprotein 4
NCBI Official Symbol:ZP4  
NCBI Official Synonym Symbols:ZBP; ZP1; ZPB; Zp-4  
NCBI Protein Information:zona pellucida sperm-binding protein 4
UniProt Protein Name:Zona pellucida sperm-binding protein 4
UniProt Synonym Protein Names:Zona pellucida glycoprotein 4; Zp-4
Protein Family:Zona pellucida sperm-binding protein
UniProt Gene Name:ZP4  
UniProt Entry Name:ZP4_HUMAN
Anti-ZP4 Antibody (CAB15180)Immunohistochemistry of paraffin-embedded Rat ovary using ZP4 Rabbit pAb (CAB15180) at dilution of 1:100 (40x lens).
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