Antibody Name:	Anti-ZP2 Antibody
Antibody SKU:	CAB10126
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 651-745 of human ZP2 (NP_003451.1).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:4000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, HL-60, SW620, Mouse liver, Rat ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 651-745 of human ZP2 (NP_003451.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KMTV SLPG PILL LSDD SSFR GVGS SDLK ASGS SGEK SRSE TGEE VGSR GAMD TKGH KTAG DVGS KAVA AVAA FAGV VATL GFIY YLYE KRTV SNH
Gene ID:	7783
Uniprot:	Q05996
Cellular Location:	Cell membrane, Secreted, Single-pass type I membrane protein, extracellular matrix, extracellular space
Calculated MW:	81kDa/82kDa
Observed MW:	68kDa

Synonyms:

ZP2, ZPA, Zp-2

Background:

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ZP2: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor. Belongs to the ZP domain family. ZPA subfamily.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 16p12 Cellular Component: endoplasmic reticulum; extracellular matrix; extracellular region; Golgi apparatus; integral to membrane; multivesicular body; plasma membrane; proteinaceous extracellular matrix; secretory granule Molecular Function:acrosin binding; coreceptor activity Biological Process: binding of sperm to zona pellucida; intracellular protein transport; manganese ion transport; regulation of acrosome reaction; signal transduction
NCBI Summary:	The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Code:	Q05996
NCBI GenInfo Identifier:	4508045
NCBI Gene ID:	7783
NCBI Accession:	NP_003451.1
UniProt Related Accession:	Q05996
Molecular Weight:
NCBI Full Name:	zona pellucida sperm-binding protein 2 isoform 1 preproprotein
NCBI Synonym Full Names:	zona pellucida glycoprotein 2
NCBI Official Symbol:	ZP2
NCBI Official Synonym Symbols:	ZPA; Zp-2; OOMD6
NCBI Protein Information:	zona pellucida sperm-binding protein 2
UniProt Protein Name:	Zona pellucida sperm-binding protein 2
UniProt Synonym Protein Names:	Zona pellucida glycoprotein 2; Zp-2
Protein Family:	Zona pellucida sperm-binding protein
UniProt Gene Name:	ZP2
UniProt Entry Name:	ZP2_HUMAN

	Western blot analysis of extracts of various cell lines, using ZP2 antibody (CAB10126) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of rat oophoroma cells using ZP2 antibody (CAB10126) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse oophoroma cells using ZP2 antibody (CAB10126) at dilution of 1:100. Blue: DAPI for nuclear staining.