Epigenetics & Nuclear Signaling Antibodies 4

Anti-ZCCHC8 Antibody (CAB9563)

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SKU:
CAB9563
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-ZCCHC8 Antibody
Antibody SKU:CAB9563
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ZCCHC8 (NP_060082.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:293T, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ZCCHC8 (NP_060082.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAE VYFG DLEL FEPF DHPE ESIP KPVH TRFK DDDG DEED ENGV GDAE LRER LRQC EETI EQLR AENQ ELKR KLNI LTRP SGIL VNDT KLDG PILQ ILFM NNAI SKQY HQEI EEFV SNLV KRFE EQQK NDVE KTSF NLLP QPSS IVLE EDHK VEES CAIK NNKE AFSV VGSV LYFT NFCL DKLG QPLL NENP QLSE GWEI PKYH QVFS HIVS LEGQ EIQV KAKR PKPH CFNC GSEE HQMK
Gene ID:55596
Uniprot:Q6NZY4
Cellular Location:Nucleus, nucleoplasm
Calculated MW:51kDa/78kDa
Observed MW:78kDa
Synonyms:ZCCHC8
Background:This gene encodes a scaffold protein which serves as an assessory factor to the nuclear RNA exosome complex. The encoded protein forms a trimeric human nuclear exosome targeting (NEXT) complex, together with hMTR4 and the RNA-binding factor RBM7 which promotes the exosomal degradation of non-coding promoter-upstream transcripts, enhancer RNAs and 3'-extended products of histone- and small nuclear RNA transcription. This complex is also thought to recruit the exosome to degrade intronic RNAs via its interaction with both the exosome and the spliceosome. It contains both an N-terminal zinc-knuckle domain and a C-terminal proline-rich domain.
UniProt Protein Function:ZCCHC8: May be involved in pre-mRNA splicing. Belongs to the ZCCHC8 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Spliceosome

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleus

Molecular Function:protein binding

Biological Process: nuclear mRNA splicing, via spliceosome

UniProt Code:Q6NZY4
NCBI GenInfo Identifier:66774213
NCBI Gene ID:55596
NCBI Accession:Q6NZY4.2
UniProt Secondary Accession:Q6NZY4,Q7L2P6, Q8N2K5, Q96SK7, Q9NSS2, Q9NSS3,
UniProt Related Accession:Q6NZY4
Molecular Weight:51,130 Da
NCBI Full Name:Zinc finger CCHC domain-containing protein 8
NCBI Synonym Full Names:zinc finger CCHC-type containing 8
NCBI Official Symbol:ZCCHC8  
NCBI Protein Information:zinc finger CCHC domain-containing protein 8
UniProt Protein Name:Zinc finger CCHC domain-containing protein 8
UniProt Synonym Protein Names:TRAMP-like complex RNA-binding factor ZCCHC8
Protein Family:Zinc finger CCHC domain-containing protein
UniProt Gene Name:ZCCHC8  
UniProt Entry Name:ZCHC8_HUMAN
Anti-ZCCHC8 Polyclonal Antibody (CAB9563)Western blot analysis of extracts of various cell lines, using ZCCHC8 antibody (CAB9563) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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