Antibody Name:	Anti-YWHAH Antibody
Antibody SKU:	CAB9079
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human YWHAH (NP_003396.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, LO2, Raji, Jurkat, Mouse heart, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human YWHAH (NP_003396.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGDR EQLL QRAR LAEQ AERY DDMA SAMK AVTE LNEP LSNE DRNL LSVA YKNV VGAR RSSW RVIS SIEQ KTMA DGNE KKLE KVKA YREK IEKE LETV CNDV LSLL DKFL IKNC NDFQ YESK VFYL KMKG DYYR YLAE VASG EKKN SVVE ASEA AYKE AFEI SKEQ MQPT HPIR LGLA LNFS VFYY EIQN APEQ ACLL AKQA FDDA IAEL DTLN EDSY KDST LIMQ LLRD NLTL WTSD QQDE EAGE GN
Gene ID:	7533
Uniprot:	Q04917
Cellular Location:
Calculated MW:	28kDa
Observed MW:	28kDa

Synonyms:

YWHAH, YWHA1

Background:

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder.

UniProt Protein Function:	14-3-3 eta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Adaptor/scaffold Chromosomal Location of Human Ortholog: 22q12.3 Cellular Component: cytoplasmic vesicle membrane; cytoplasm; plasma membrane; cytosol Molecular Function:insulin-like growth factor receptor binding; protein domain specific binding; protein binding; enzyme binding; sodium channel regulator activity; protein heterodimerization activity; actin binding; glucocorticoid receptor binding Biological Process: intracellular protein transport; substantia nigra development; regulation of neuron differentiation; apoptosis; glucocorticoid receptor signaling pathway; positive regulation of transcription, DNA-dependent; regulation of synaptic plasticity; glucocorticoid catabolic process; negative regulation of dendrite morphogenesis; regulation of sodium ion transport
NCBI Summary:	This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]
UniProt Code:	Q04917
NCBI GenInfo Identifier:	4507951
NCBI Gene ID:	7533
NCBI Accession:	NP_003396.1
UniProt Related Accession:	Q04917
Molecular Weight:	28,219 Da
NCBI Full Name:	14-3-3 protein eta
NCBI Synonym Full Names:	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
NCBI Official Symbol:	YWHAH
NCBI Official Synonym Symbols:	YWHA1
NCBI Protein Information:	14-3-3 protein eta; 14-3-3 eta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
UniProt Protein Name:	14-3-3 protein eta
UniProt Synonym Protein Names:	Protein AS1
Protein Family:	14-3-3 protein
UniProt Gene Name:	YWHAH
UniProt Entry Name:	1433F_HUMAN

	Western blot analysis of extracts of various cell lines, using YWHAH antibody (CAB9079) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of NIH-3T3 cells using Anti-YWHAH Polyclonal Antibody (CAB9079) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using Anti-YWHAH Polyclonal Antibody (CAB9079) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.