Immunology Antibodies 1
Anti-YTHDF1 Antibody (CAB13260)
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- SKU:
- CAB13260
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-YTHDF1 Antibody |
| Antibody SKU: | CAB13260 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human YTHDF1 (NP_060268.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human YTHDF1 (NP_060268.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSAT SVDT QRTK GQDN KVQN GSLH QKDT VHDN DFEP YLTG QSNQ SNSY PSMS DPYL SSYY PPSI GFPY SLNE APWS TAGD PPIP YLTT YGQL SNGD HHFM HDAV FGQP GGLG NNIY QHRF NFFP ENPA FSAW GTSG SQGQ QTQS SAYG SSYT YPPS SLGG TVVD GQPG FHSD TLSK APGM NSLE QGMV GLKI GDVS SSAV |
| Gene ID: | 54915 |
| Uniprot: | Q9BYJ9 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 20kDa/60kDa |
| Observed MW: | 61kDa |
| Synonyms: | YTHDF1, C20orf21 |
| Background: |
| UniProt Protein Function: | YTHDF1: 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA-binding Chromosomal Location of Human Ortholog: 20q13.33 |
| UniProt Code: | Q9BYJ9 |
| NCBI GenInfo Identifier: | 28380041 |
| NCBI Gene ID: | 54915 |
| NCBI Accession: | Q9BYJ9.1 |
| UniProt Secondary Accession: | Q9BYJ9,Q8N3G5, Q8TBT1, Q96AN4, Q96S57, Q9BTI7, Q9NX79 |
| UniProt Related Accession: | Q9BYJ9 |
| Molecular Weight: | 61kDa |
| NCBI Full Name: | YTH domain-containing family protein 1 |
| NCBI Synonym Full Names: | YTH N(6)-methyladenosine RNA binding protein 1 |
| NCBI Official Symbol: | YTHDF1 |
| NCBI Official Synonym Symbols: | C20orf21 |
| NCBI Protein Information: | YTH domain-containing family protein 1; DACA-1; YTH domain family 1; YTH domain family protein 1; YTH domain family, member 1; dermatomyositis associated with cancer putative autoantigen 1 |
| UniProt Protein Name: | YTH domain-containing family protein 1 |
| UniProt Synonym Protein Names: | Dermatomyositis associated with cancer putative autoantigen 1; DACA-1 |
| Protein Family: | YTH domain-containing family protein |
| UniProt Gene Name: | YTHDF1 |
| UniProt Entry Name: | YTHD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using YTHDF1 antibody (CAB13260) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of L929 cells using YTHDF1 Rabbit pAb (CAB13260) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using YTHDF1 Rabbit pAb (CAB13260) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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