Antibody Name:	Anti-YB1 Antibody
Antibody SKU:	CAB3534
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human YB1

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis, Rat heart, HeLa

Immunogen:	A synthesized peptide derived from human YB1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4904
Uniprot:	P67809
Cellular Location:
Calculated MW:	50kDa
Observed MW:	49KDa

Synonyms:

BP-8, CBF-A, CSDA2, CSDB, DBPB, EFI-A, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1

Background:

This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015]

UniProt Protein Function:	YB-1: a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Involved in cisplatin resistance. Seems to be a negative regulatory factor. May play a role in both transcriptional and translational regulation of spermatogenesis. Found at very low levels at day 10 and levels increase at day 15 and persist throughout adulthood.
UniProt Protein Details:	Protein type:Transcription factor; RNA-binding; RNA splicing; RNA processing Chromosomal Location of Human Ortholog: 1p34 Cellular Component: nucleoplasm; nuclear membrane; intracellular membrane-bound organelle; stress granule; cytoplasm; nucleus; ribonucleoprotein complex; U12-dependent spliceosome Molecular Function:protein binding; DNA binding; RNA binding; double-stranded DNA binding; transcription factor activity; single-stranded DNA binding Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; negative regulation of striated muscle cell differentiation; regulation of transcription, DNA-dependent; in utero embryonic development; positive regulation of cell division; RNA splicing; positive regulation of transcription from RNA polymerase II promoter; gene expression; negative regulation of transcription from RNA polymerase II promoter
UniProt Code:	P67809
NCBI GenInfo Identifier:	34098946
NCBI Gene ID:	4904
NCBI Accession:	NP_004550.2
UniProt Secondary Accession:	P67809,P16990, P16991, Q14972, Q15325, Q5FVF0,
UniProt Related Accession:	P67809
Molecular Weight:	35,924 Da
NCBI Full Name:	nuclease-sensitive element-binding protein 1
NCBI Synonym Full Names:	Y box binding protein 1
NCBI Official Symbol:	YBX1
NCBI Official Synonym Symbols:	YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1
NCBI Protein Information:	nuclease-sensitive element-binding protein 1; CBF-A; EFI-A; DNA-binding protein B; Y-box-binding protein 1; Y-box transcription factor; enhancer factor I subunit A; nuclease sensitive element binding protein 1; CCAAT-binding transcription factor I subunit
UniProt Protein Name:	Nuclease-sensitive element-binding protein 1
UniProt Synonym Protein Names:	CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1
Protein Family:	Nuclease-sensitive element-binding protein
UniProt Gene Name:	YBX1
UniProt Entry Name:	YBOX1_HUMAN

	Western blot analysis of extracts of HeLa cells, using YB-1/YBX1 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using YB-1/YBX1 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat brain using YB-1/YBX1 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human oophoroma using YB-1/YBX1 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded mouse brain using YB-1/YBX1 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
	Immunofluorescence analysis of C6 cells using YB-1/YBX1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using YB-1/YBX1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using YB-1/YBX1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug YB-1/YBX1 antibody at a dilition of 1:1000.
	Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using YB-1/YBX1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.