Signal Transduction Antibodies 1

Anti-XPOT Antibody (CAB12043)

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SKU:
CAB12043
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-XPOT Antibody
Antibody SKU:CAB12043
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human XPOT (NP_009166.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:B cells, Mouse brain, Mouse kidney, Mouse liver, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human XPOT (NP_009166.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDEQ ALLG LNPN ADSD FRQR ALAY FEQL KISP DAWQ VCAE ALAQ RTYS DDHV KFFC FQVL EHQV KYKY SELT TVQQ QLIR ETLI SWLQ AQML NPQP EKTF IRNK AAQV FALL FVTE YLTK WPKF FFDI LSVV DLNP RGVD LYLR ILMA IDSE LVDR DVVH TSEE ARRN TLIK DTMR EQCI PNLV ESWY QILQ NYQF TNSE
Gene ID:11260
Uniprot:O43592
Cellular Location:Cytoplasm, Nucleus
Calculated MW:109kDa
Observed MW:100kDa
Synonyms:XPOT, XPO3
Background:This gene encodes a protein belonging to the RAN-GTPase exportin family that mediates export of tRNA from the nucleus to the cytoplasm. Translocation of tRNA to the cytoplasm occurs once exportin has bound both tRNA and GTP-bound RAN.
UniProt Protein Function:exportin T: Mediates the nuclear export of aminoacylated tRNAs. In the nucleus binds to tRNA and to the GTPase Ran in its active GTP- bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the tRNA from the export receptor. XPOT then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the exportin family.
UniProt Protein Details:

Protein type:Karyopherin; Nuclear export; Nuclear import

Chromosomal Location of Human Ortholog: 12q14.2

Cellular Component: cytoplasm; cytosol; nuclear matrix; nuclear pore; nucleoplasm

Molecular Function:nucleobase, nucleoside, nucleotide and nucleic acid transmembrane transporter activity; protein binding; Ran GTPase binding; tRNA binding

Biological Process: tRNA export from nucleus

NCBI Summary:This gene encodes a protein belonging to the RAN-GTPase exportin family that mediates export of tRNA from the nucleus to the cytoplasm. Translocation of tRNA to the cytoplasm occurs once exportin has bound both tRNA and GTP-bound RAN. [provided by RefSeq, Jul 2008]
UniProt Code:O43592
NCBI GenInfo Identifier:108862045
NCBI Gene ID:11260
NCBI Accession:O43592.2
UniProt Secondary Accession:O43592,O43784, Q8WUG2, Q9BVS7, A6NLH1,
UniProt Related Accession:O43592
Molecular Weight:110kDa
NCBI Full Name:Exportin-T
NCBI Synonym Full Names:exportin for tRNA
NCBI Official Symbol:XPOT  
NCBI Official Synonym Symbols:XPO3  
NCBI Protein Information:exportin-T
UniProt Protein Name:Exportin-T
UniProt Synonym Protein Names:Exportin(tRNA); tRNA exportin
UniProt Gene Name:XPOT  
Anti-XPOT Antibody (CAB12043)Western blot analysis of extracts of various cell lines, using XPOT antibody (CAB12043) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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