KO Validated Antibodies 1

Anti-XIAP Antibody (CAB6869)[KO Validated]

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SKU:
CAB6869
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-XIAP Antibody [KO Validated]
Antibody SKU:CAB6869
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human XIAP (NP_001158.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF-7, HeLa, SW620, HepG2, Jurkat, Mouse liver, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human XIAP (NP_001158.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTFN SFEG SKTC VPAD INKE EEFV EEFN RLKT FANF PSGS PVSA STLA RAGF LYTG EGDT VRCF SCHA AVDR WQYG DSAV GRHR KVSP NCRF INGF YLEN SATQ STNS GIQN GQYK VENY LGSR DHFA LDRP SETH ADYL LRTG QVVD ISDT IYPR NPAM YSEE ARLK SFQN WPDY AHLT PREL ASAG LYYT GIGD QVQC
Gene ID:331
Uniprot:P98170
Cellular Location:Cytoplasm, Nucleus
Calculated MW:56kDa
Observed MW:54kDa
Synonyms:XIAP, API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3
Background:This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.
UniProt Protein Function:XIAP: an apoptotic suppressor protein. Inhibist apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. Inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. Inhibits cell-death proteases, caspase-3, caspase-7, and perhaps caspase-9.
UniProt Protein Details:

Protein type:EC 6.3.2.-; Apoptosis; Ubiquitin ligase; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: nucleoplasm; spindle microtubule; cytoplasm; nucleus; cytosol

Molecular Function:protein binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: Wnt receptor signaling pathway; positive regulation of protein ubiquitination; apoptosis; regulation of inflammatory response; regulation of BMP signaling pathway; protein ubiquitination; negative regulation of caspase activity; response to DNA damage stimulus; copper ion homeostasis; regulation of cell proliferation; negative regulation of apoptosis; regulation of innate immune response

Disease: Lymphoproliferative Syndrome, X-linked, 1; Lymphoproliferative Syndrome, X-linked, 2

NCBI Summary:This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011]
UniProt Code:P98170
NCBI GenInfo Identifier:12643387
NCBI Gene ID:331
NCBI Accession:P98170.2
UniProt Secondary Accession:P98170,Q9NQ14, D3DTF2,
UniProt Related Accession:P98170
Molecular Weight:497
NCBI Full Name:E3 ubiquitin-protein ligase XIAP
NCBI Synonym Full Names:X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase
NCBI Official Symbol:XIAP  
NCBI Official Synonym Symbols:API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3  
NCBI Protein Information:E3 ubiquitin-protein ligase XIAP; X-linked IAP; IAP-like protein; inhibitor of apoptosis protein 3; baculoviral IAP repeat-containing protein 4
UniProt Protein Name:E3 ubiquitin-protein ligase XIAP
UniProt Synonym Protein Names:Baculoviral IAP repeat-containing protein 4; IAP-like protein; ILP; hILP; Inhibitor of apoptosis protein 3; IAP-3; hIAP-3; hIAP3; X-linked inhibitor of apoptosis protein; X-linked IAP
Protein Family:XIAP-associated factor
UniProt Gene Name:XIAP  
UniProt Entry Name:XIAP_HUMAN
Anti-XIAP Antibody [KO Validated] (CAB6869)Western blot analysis of extracts of various cell lines, using XIAP antibody (CAB6869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-XIAP Antibody [KO Validated] (CAB6869)Western blot analysis of extracts from normal (control) and XIAP knockout (KO) HeLa cells, using XIAP antibody (CAB6869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-XIAP Antibody [KO Validated] (CAB6869)Immunohistochemistry of paraffin-embedded human kidney using XIAP antibody (CAB6869) at dilution of 1:100 (40x lens).
Anti-XIAP Antibody [KO Validated] (CAB6869)Immunohistochemistry of paraffin-embedded mouse kidney using XIAP antibody (CAB6869) at dilution of 1:100 (40x lens).
Anti-XIAP Antibody [KO Validated] (CAB6869)Immunofluorescence analysis of MCF7 cells using XIAP antibody (CAB6869).
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