Cell Biology Antibodies 12

Anti-VPS28 Antibody (CAB9104)

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SKU:
CAB9104
Product Type:
Antibody
Applications:
WB
Applications:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-VPS28 Antibody
Antibody SKU:CAB9104
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human VPS28 (NP_057292.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, U-251MG, 293T, HepG2, Mouse brain, Mouse kidney, Mouse liver, Mouse spleen, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human VPS28 (NP_057292.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MFHG IPAT PGIG APGN KPEL YEEV KLYK NARE REKY DNMA ELFA VVKT MQAL EKAY IKDC VSPS EYTA ACSR LLVQ YKAA FRQV QGSE ISSI DEFC RKFR LDCP LAME RIKE DRPI TIKD DKGN LNRC IADV VSLF ITVM DKLR LEIR AMDE IQPD LREL METM HRMS HLPP DFEG RQTV SQWL QTLS GMSA SDEL DDSQ VRQM LFDL ESAY NAFN RFLH A
Gene ID:51160
Uniprot:Q9UK41
Cellular Location:Cell membrane, Late endosome membrane, Peripheral membrane protein
Calculated MW:25kDa/26kDa
Observed MW:25kDa
Synonyms:VPS28
Background:This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
NCBI Summary:This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
UniProt Code:Q9UK41
NCBI GenInfo Identifier:7705885
NCBI Gene ID:51160
NCBI Accession:NP_057292
UniProt Secondary Accession:Q9UK41,Q86VK0,
UniProt Related Accession:Q9UK41
Molecular Weight:25.4 kDa (221aa), confirmed by MALDI-TOF.
NCBI Full Name:vacuolar protein sorting-associated protein 28 homolog isoform 1
NCBI Synonym Full Names:VPS28, ESCRT-I subunit
NCBI Official Symbol:VPS28  
NCBI Protein Information:vacuolar protein sorting-associated protein 28 homolog
UniProt Protein Name:Vacuolar protein sorting-associated protein 28 homolog
UniProt Synonym Protein Names:ESCRT-I complex subunit VPS28
Protein Family:Vacuolar protein sorting-associated protein
UniProt Gene Name:VPS28  
Anti-VPS28 Polyclonal Antibody (CAB9104)Western blot analysis of extracts of various cell lines, using VPS28 antibody (CAB9104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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