Signal Transduction Antibodies 1

Anti-VISTA Antibody (CAB10419)

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SKU:
CAB10419
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-VISTA Antibody
Antibody SKU:CAB10419
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 33-194 of human VISTA (NP_071436.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa, LO2, A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 33-194 of human VISTA (NP_071436.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FKVA TPYS LYVC PEGQ NVTL TCRL LGPV DKGH DVTF YKTW YRSS RGEV QTCS ERRP IRNL TFQD LHLH HGGH QAAN TSHD LAQR HGLE SASD HHGN FSIT MRNL TLLD SGLY CCLV VEIR HHHS EHRV HGAM ELQV QTGK DAPS NCVV YPSS SQDS ENIT AA
Gene ID:64115
Uniprot:Q9H7M9
Cellular Location:Cell membrane, Single-pass type I membrane protein
Calculated MW:33kDa
Observed MW:37kDa
Synonyms:VSIR, B7-H5, B7H5, C10orf54, DD1alpha, GI24, PD-1H, PP2135, SISP1, VISTA
Background:
UniProt Protein Function:VISTA: May stimulate MMP14-mediated MMP2 activation
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: cell surface; intercellular junction; plasma membrane

Biological Process: cell-cell adhesion

UniProt Code:Q9H7M9
NCBI GenInfo Identifier:62339432
NCBI Gene ID:64115
NCBI Accession:NP_071436.1
UniProt Related Accession:Q9H7M9
Molecular Weight:34kDa
NCBI Full Name:V-type immunoglobulin domain-containing suppressor of T-cell activation
NCBI Synonym Full Names:V-set immunoregulatory receptor
NCBI Official Symbol:VSIR  
NCBI Official Synonym Symbols:B7H5; GI24; B7-H5; Dies1; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha  
NCBI Protein Information:V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Protein Name:V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Synonym Protein Names:Platelet receptor Gi24
UniProt Gene Name:C10orf54  
UniProt Entry Name:VISTA_HUMAN
Anti-VISTA Antibody (CAB10419)Western blot analysis of extracts of various cell lines, using VISTA antibody (CAB10419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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