KO Validated Antibodies 2

Anti-Vimentin Antibody [KO Validated] (CAB19607)

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SKU:
CAB19607
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-Vimentin Antibody [KO Validated]
Antibody SKU:CAB19607
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Vimentin.
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Jurkat, HeLa, Mouse testis, C6
Immunogen:A synthesized peptide derived from human Vimentin.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:7431
Uniprot:P08670
Cellular Location:Cytoplasm
Calculated MW:54kDa
Observed MW:54kDa
Anti-Vimentin Antibody [KO Validated] (CAB19607)Western blot analysis of extracts of various cell lines, using Vimentin antibody (CAB19607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-Vimentin Antibody [KO Validated] (CAB19607)Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (CAB19607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-Vimentin Antibody [KO Validated] (CAB19607)Immunohistochemistry of paraffin-embedded Rat spleen using Vimentin antibody (CAB19607) at dilution of 1:100 (40x lens).
Anti-Vimentin Antibody [KO Validated] (CAB19607)Immunohistochemistry of paraffin-embedded Human colon carcinoma using Vimentin antibody (CAB19607) at dilution of 1:100 (40x lens).
Anti-Vimentin Antibody [KO Validated] (CAB19607)Immunohistochemistry of paraffin-embedded Mouse kidney using Vimentin antibody (CAB19607) at dilution of 1:100 (40x lens).
Synonyms:CTRCT30, HEL113, Vimentin, VIM, vimentin
Background:This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
UniProt Protein Function:Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
UniProt Protein Details:

Protein type:Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function:protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding

Disease: Cataract 30

NCBI Summary:This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
UniProt Code:P08670
NCBI GenInfo Identifier:55977767
NCBI Gene ID:7431
NCBI Accession:P08670.4
UniProt Related Accession:P08670
Molecular Weight:
NCBI Full Name:Vimentin
NCBI Synonym Full Names:vimentin
NCBI Official Symbol:VIM  
NCBI Protein Information:vimentin
UniProt Protein Name:Vimentin
Protein Family:Vimentin
UniProt Gene Name:VIM  
UniProt Entry Name:VIME_HUMAN
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