Cell Death Antibodies 2
Anti-VIL-1 Antibody (CAB5494)
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- SKU:
- CAB5494
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | VIL1 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB5494 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human VIL1 (NP_009058.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 22Rv1, K-562, HepG2, HT-29, MCF7, Mouse kidney, Mouse intestine |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human VIL1 (NP_009058.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTKL SAQV KGSL NITT PGLQ IWRI EAMQ MVPV PSST FGSF FDGD CYII LAIH KTAS SLSY DIHY WIGQ DSSL DEQG AAAI YTTQ MDDF LKGR AVQH REVQ GNES EAFR GYFK QGLV IRKG GVAS GMKH VETN SYDV QRLL HVKG KRNV VAGE VEMS WKSF NRGD VFLL DLGK LIIQ WNGP ESTR MERL RGMT LAKE IRDQ ERGG RTYV GVVD GENE LASP KLME VMNH VLGK RREL KAAV PDTV VEPA LKAA LKLY HVSD SEGN LVVR EVAT RPLT QDLL SHED CYIL DQGG LKIY VWKG KKAN EQEK KGAM SHAL NFIK AKQY PPST QVEV QNDG AESA VFQQ LFQK WT |
| Gene ID: | 7429 |
| Uniprot: | P09327 |
| Cellular Location: | Cell projection, Cytoplasm, cytoskeleton, filopodium, filopodium tip, lamellipodium, microvillus, ruffle |
| Calculated MW: | 46kDa/92kDa |
| Observed MW: | 100kDa |
| Synonyms: | VIL1, D2S1471, VIL, villin-1 |
| Background: | This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. |
| UniProt Protein Function: | Villin: Epithelial cell-specific Ca(2+)-regulated actin- modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. Monomer. Homodimer; homodimerization is necessary for actin-bundling. Associates with F-actin; phosphorylation at tyrosines residues decreases the association with F-actin. Interacts (phosphorylated at C-terminus tyrosine phosphorylation sites) with PLCG1 (via the SH2 domains). Interacts (phosphorylated form) with PLCG1; the interaction is enhanced by hepatocyte growth factor (HGF). Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes. Belongs to the villin/gelsolin family. |
| UniProt Protein Details: | Protein type:Actin-binding; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2q35 Cellular Component: ruffle; filopodium tip; microvillus; lamellipodium; cytoplasm; actin filament bundle; filopodium Molecular Function:actin filament binding; identical protein binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein homodimerization activity; caspase inhibitor activity; calcium ion binding Biological Process: epidermal growth factor receptor signaling pathway; actin filament severing; actin filament depolymerization; actin filament capping; negative regulation of caspase activity; actin nucleation; regulation of cell shape; response to bacterium; epithelial cell differentiation; actin filament polymerization; regulation of actin nucleation; positive regulation of actin filament bundle formation; protein complex assembly; positive regulation of cell migration |
| NCBI Summary: | This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P09327 |
| NCBI GenInfo Identifier: | 224471905 |
| NCBI Gene ID: | 7429 |
| NCBI Accession: | P09327.4 |
| UniProt Secondary Accession: | P09327,Q53S11, Q96AC8, B2R9A7, |
| UniProt Related Accession: | P09327 |
| Molecular Weight: | 827 |
| NCBI Full Name: | Villin-1 |
| NCBI Synonym Full Names: | villin 1 |
| NCBI Official Symbol: | VIL1 |
| NCBI Official Synonym Symbols: | VIL; D2S1471 |
| NCBI Protein Information: | villin-1 |
| UniProt Protein Name: | Villin-1 |
| Protein Family: | VIN3-like protein |
| UniProt Gene Name: | VIL1 |
| UniProt Entry Name: | VILI_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VIL1 antibody (CAB5494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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