Cell Biology Antibodies 7

Anti-VCL Antibody (CAB1758)

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SKU:
CAB1758
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-VCL Antibody
Antibody SKU:CAB1758
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 767-1066 of human VCL (NP_003364.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, PC-12, Mouse heart, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 767-1066 of human VCL (NP_003364.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AKRE VENS EDPK FREA VKAA SDEL SKTI SPMV MDAK AVAG NISD PGLQ KSFL DSGY RILG AVAK VREA FQPQ EPDF PPPP PDLE QLRL TDEL APPK PPLP EGEV PPPR PPPP EEKD EEFP EQKA GEVI NQPM MMAA RQLH DEAR KWSS KGND IIAA AKRM ALLM AEMS RLVR GGSG TKRA LIQC AKDI AKAS DEVT RLAK EVAK QCTD KRIR TNLL QVCE RIPT ISTQ LKIL STVK ATML GRTN ISDE ESEQ ATEM LVHN AQNL MQSV KETV REAE AASI KIRT DAGF TLRW VRKT PWYQ
Gene ID:7414
Uniprot:P18206
Cellular Location:Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton, focal adhesion
Calculated MW:24kDa/116kDa/123kDa
Observed MW:140kDa
Synonyms:VCL, CMD1W, CMH15, HEL114, MV, MVCL, vinculin
Background:Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
UniProt Protein Function:Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell adhesion; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: cytoskeleton; adherens junction; protein complex; cell-cell adherens junction; focal adhesion; costamere; plasma membrane; extracellular region; fascia adherens; intercellular junction; cytosol; vesicle; cell-matrix junction; actin cytoskeleton

Molecular Function:protein binding; cadherin binding; beta-catenin binding; structural molecule activity; actin binding; alpha-catenin binding

Biological Process: lamellipodium biogenesis; platelet activation; platelet degranulation; apical junction assembly; muscle contraction; axon extension; cell-matrix adhesion; morphogenesis of an epithelium; cell adhesion; cell motility; blood coagulation; negative regulation of cell migration

Disease: Cardiomyopathy, Dilated, 1w

NCBI Summary:Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:P18206
NCBI GenInfo Identifier:21903479
NCBI Gene ID:7414
NCBI Accession:P18206.4
UniProt Secondary Accession:P18206,Q16450, Q5SWX2, Q7Z3B8, Q8IXU7,
UniProt Related Accession:P18206
Molecular Weight:24,904 Da
NCBI Full Name:Vinculin
NCBI Synonym Full Names:vinculin
NCBI Official Symbol:VCL  
NCBI Official Synonym Symbols:MV; MVCL; CMD1W; CMH15; HEL114  
NCBI Protein Information:vinculin; metavinculin; epididymis luminal protein 114
UniProt Protein Name:Vinculin
UniProt Synonym Protein Names:Metavinculin; MV
Protein Family:Vinculin
UniProt Gene Name:VCL  
UniProt Entry Name:VINC_HUMAN
Anti-VCL Antibody (CAB1758)Western blot analysis of extracts of various cell lines, using VCL antibody (CAB1758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-VCL Antibody (CAB1758)Immunohistochemistry of paraffin-embedded human smooth muscle using VCL Antibody (CAB1758) at dilution of 1:100 (40x lens).
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