Cell Biology Antibodies 14

Anti-VCAM1 Antibody (CAB16999)

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SKU:
CAB16999
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-VCAM1 Antibody (CAB16999)
Antibody SKU:CAB16999
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence, Immunoprecipitation
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 480-630 of human VCAM1 (NP_001069.1).
Application:Western blotting, Immunofluorescence, Immunoprecipitation
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, RAW264.7, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 480-630 of human VCAM1 (NP_001069.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ALVC QAKL HIDD MEFE PKQR QSTQ TLYV NVAP RDTT VLVS PSSI LEEG SSVN MTCL SQGF PAPK ILWS RQLP NGEL QPLS ENAT LTLI STKM EDSG VYLC EGIN QAGR SRKE VELI IQVT PKDI KLTA FPSE SVKE GDTV IISC TCGN VPE
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:71kDa/74kDa/81kDa
Observed MW:85-100KDa
Synonyms:CD106, INCAM-100, VCAM1
Background:This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
Western blot analysis of extracts of RAW264. 7 cells, using VCAM1 antibody at 1:500 dilution. RAW264. 7 cells were treated by TNF-α (20 ng/ml) at 37℃ for 16 hours after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of RAW264. 7 cells, using VCAM1 antibody at 1:500 dilution. RAW264. 7 cells were treated by TNF-α (20 ng/ml) at 37℃ for 16 hours after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Western blot analysis of extracts of various cell lines, using VCAM1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Western blot analysis of extracts of various cell lines, using VCAM1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
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