Cell Biology Antibodies 1

Anti-VCAM1 Antibody (CAB0279)

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SKU:
CAB0279
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-VCAM1 Antibody
Antibody SKU:CAB0279
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300-400 of human VCAM1 (NP_001069.1).
Application:WB IHC IP
Recommended Dilution:WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HeLa
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300-400 of human VCAM1 (NP_001069.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NRKE VELI VQEK PFTV EISP GPRI AAQI GDSV MLTC SVMG CESP SFSW RTQI DSPL SGKV RSEG TNST LTLS PVSF ENEH SYLC TVTC GHKK LEKG IQVE L
Gene ID:7412
Uniprot:P19320
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:71kDa/74kDa/81kDa
Observed MW:103kDa
Synonyms:CD106, INCAM-100, VCAM1
Background:This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
UniProt Protein Function:VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: Golgi apparatus; extracellular space; cell surface; microvillus; endoplasmic reticulum; early endosome; integral to membrane; apical part of cell; plasma membrane; podosome; sarcolemma; filopodium; external side of plasma membrane

Molecular Function:amine oxidase activity; integrin binding; cell adhesion molecule binding

Biological Process: response to nicotine; regulation of immune response; chronic inflammatory response; extracellular matrix organization and biogenesis; viral reproduction; heart development; cytokine and chemokine mediated signaling pathway; cell-matrix adhesion; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; B cell differentiation; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; amine metabolic process; response to ionizing radiation; cell adhesion; leukocyte tethering or rolling; acute inflammatory response; response to nutrient; aging

NCBI Summary:This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]
UniProt Code:P19320
NCBI GenInfo Identifier:137560
NCBI Gene ID:7412
NCBI Accession:P19320.1
UniProt Secondary Accession:P19320,Q6NUP8, A8K6R7, B4DKS4, E9PDD1,
UniProt Related Accession:P19320
Molecular Weight:74,346 Da
NCBI Full Name:Vascular cell adhesion protein 1
NCBI Synonym Full Names:vascular cell adhesion molecule 1
NCBI Official Symbol:VCAM1  
NCBI Official Synonym Symbols:CD106; INCAM-100  
NCBI Protein Information:vascular cell adhesion protein 1; CD106 antigen
UniProt Protein Name:Vascular cell adhesion protein 1
UniProt Synonym Protein Names:INCAM-100; CD_antigen: CD106
Protein Family:Vascular cell adhesion protein
UniProt Gene Name:VCAM1  
UniProt Entry Name:VCAM1_HUMAN
Anti-VCAM1 Antibody (CAB0279)Western blot analysis of extracts of various cell lines, using VCAM1 Antibody (CAB0279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-VCAM1 Antibody (CAB0279)Immunohistochemistry of paraffin-embedded Human lung cancer using VCAM1 antibody (CAB0279) at dilution of 1:100 (40x lens).
Anti-VCAM1 Antibody (CAB0279)Immunohistochemistry of paraffin-embedded Human stomach using VCAM1 antibody (CAB0279) at dilution of 1:100 (40x lens).
Anti-VCAM1 Antibody (CAB0279)Immunohistochemistry of paraffin-embedded Mouse stomach using VCAM1 antibody (CAB0279) at dilution of 1:100 (40x lens).
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