Developmental Biology
Anti-VAMP5 Antibody (CAB14371)
- SKU:
- CAB14371
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-VAMP5 Antibody |
| Antibody SKU: | CAB14371 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human VAMP5 (NP_006625.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human VAMP5 (NP_006625.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAGI ELER CQQQ ANEV TEIM RNNF GKVL ERGV KLAE LQQR SDQL LDMS STFN KTTQ NLAQ KKCW ENIR YRIC VGLV VVGV LLII LIVL LVVF LPQS SDSS SAPR TQDA GIAS GPGN |
| Gene ID: | 10791 |
| Uniprot: | O95183 |
| Cellular Location: | Cell membrane, Endomembrane system, Golgi apparatus, Single-pass type IV membrane protein, trans-Golgi network membrane |
| Calculated MW: | 12kDa |
| Observed MW: | 16kDa |
| Synonyms: | VAMP5 |
| Background: | Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of vesicles and cell membranes. The VAMP5 gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. This VAMP family member may participate in vesicle trafficking events that are associated with myogenesis. |
| UniProt Protein Function: | VAMP5: May participate in trafficking events that are associated with myogenesis, such as myoblast fusion and/or GLUT4 trafficking. Belongs to the synaptobrevin family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 2p11.2 Cellular Component: SNARE complex; synaptic vesicle; cytoplasmic vesicle membrane; cell surface; perinuclear region of cytoplasm; integral to plasma membrane; late endosome; plasma membrane; trans-Golgi network Molecular Function:SNAP receptor activity; SNARE binding Biological Process: vesicle fusion; muscle development; skeletal muscle development; exocytosis; neurotransmitter secretion; Golgi to plasma membrane protein transport; cell differentiation |
| NCBI Summary: | Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of vesicles and cell membranes. The VAMP5 gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. This VAMP family member may participate in vesicle trafficking events that are associated with myogenesis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95183 |
| NCBI GenInfo Identifier: | 5730112 |
| NCBI Gene ID: | 10791 |
| NCBI Accession: | NP_006625.1 |
| UniProt Secondary Accession: | O95183,Q9P0T2, |
| UniProt Related Accession: | O95183 |
| Molecular Weight: | 12,805 Da |
| NCBI Full Name: | vesicle-associated membrane protein 5 |
| NCBI Synonym Full Names: | vesicle-associated membrane protein 5 |
| NCBI Official Symbol: | VAMP5 |
| NCBI Protein Information: | vesicle-associated membrane protein 5; VAMP-5; myobrevin |
| UniProt Protein Name: | Vesicle-associated membrane protein 5 |
| UniProt Synonym Protein Names: | Myobrevin |
| Protein Family: | Vesicle-associated membrane protein |
| UniProt Gene Name: | VAMP5 |
| UniProt Entry Name: | VAMP5_HUMAN |
 | Western blot analysis of extracts of mouse spleen, using VAMP5 antibody (CAB14371) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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