Description
Antibody Name: | Anti-USP9Y Antibody (CAB20758) |
Antibody SKU: | CAB20758 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 2456-2555 of human USP9Y (NP_004645.2). |
Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | HeLa, Mouse testis, Rat brain |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 2456-2555 of human USP9Y (NP_004645.2). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | YFLE RSHS ARMT LAKA CELC PEEE PDDQ DAPD EHEP SPSE DAPL YPHS PASQ YQQN NHVH GQPY TGPA AHHL NNPQ KTGQ RTQE NYEG NEEV SSPQ MKDQ |
Cellular Location: | |
Calculated MW: | 291kDa |
Observed MW: | 291KDa |
Synonyms: | DFFRY, SPGFY2 |
Background: | This gene is a member of the peptidase C19 family. It encodes a protein that is similar to ubiquitin-specific proteases, which cleave the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. |
Immunohistochemistry of paraffin-embedded rat kidney using USP9Y Rabbit pAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded mouse kidney using USP9Y Rabbit pAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded mouse testis using USP9Y Rabbit pAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Western blot analysis of extracts of various cell lines, using USP9Y antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s. |