Developmental Biology

Anti-USP42 Antibody (CAB15911)

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SKU:
CAB15911
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-USP42 Antibody
Antibody SKU:CAB15911
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human USP42 (NP_115548.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HT-29, Raji, SGC7901, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human USP42 (NP_115548.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ASVQ NWSV NRSS VIPE HPKK QKIT ISIH NKLP VRQC QSQP NLHS NSLE NPTK PVPS STIT NSAV QSTS NAST MSVS SKVT KPIP RSES CSQP VMNG KSKL NSSV LVPY GAES SEDS DEES KGLG KENG IGTI VSSH SPGQ DAED EEAT PHEL QEPM TLNG ANSA DSDS DPKE NGLA PDGA SCQG QPAL HSEN PFAK ANGL P
Gene ID:84132
Uniprot:Q9H9J4
Cellular Location:
Calculated MW:144kDa/145kDa
Observed MW:170kDa
Synonyms:USP42
Background:
UniProt Protein Function:USP42: Deubiquitinating enzyme which may play an important role during spermatogenesis. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin-specific protease; Protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 7p22.1

Molecular Function:protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; spermatogenesis; cell differentiation

UniProt Code:Q9H9J4
NCBI GenInfo Identifier:79750944
NCBI Gene ID:84132
NCBI Accession:NP_115548.1
UniProt Secondary Accession:Q9H9J4,Q0VIN8, Q3C166, Q6P9B4, A2RUE3, B5MDA5,
UniProt Related Accession:Q9H9J4
Molecular Weight:144,335 Da
NCBI Full Name:ubiquitin carboxyl-terminal hydrolase 42
NCBI Synonym Full Names:ubiquitin specific peptidase 42
NCBI Official Symbol:USP42  
NCBI Protein Information:ubiquitin carboxyl-terminal hydrolase 42; ubiquitin thioesterase 42; deubiquitinating enzyme 42; ubiquitin thiolesterase 42; ubiquitin specific protease 42; ubiquitin-specific-processing protease 42
UniProt Protein Name:Ubiquitin carboxyl-terminal hydrolase 42
UniProt Synonym Protein Names:Deubiquitinating enzyme 42; Ubiquitin thioesterase 42; Ubiquitin-specific-processing protease 42
Protein Family:Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name:USP42  
UniProt Entry Name:UBP42_HUMAN
Anti-USP42 Antibody (CAB15911)Western blot analysis of extracts of various cell lines, using USP42 antibody (CAB15911) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 160s.
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