Signal Transduction Antibodies 1
Anti-USP4 Antibody (CAB12242)
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- SKU:
- CAB12242
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-USP4 Antibody |
| Antibody SKU: | CAB12242 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 560-700 of human USP4 (NP_003354.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, Mouse brain, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 560-700 of human USP4 (NP_003354.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DDIF VYEV CSTS VDGS ECVT LPVY FRER KSRP SSTS SASA LYGQ PLLL SVPK HKLT LESL YQAV CDRI SRYV KQPL PDEF GSSP LEPG ACNG SRNS CEGE DEEE MEHQ EEGK EQLS ETEG SGED EPGN DPSE TTQK KIKG Q |
| Gene ID: | 7375 |
| Uniprot: | Q13107 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 35kDa/103kDa/108kDa |
| Observed MW: | 95kDa |
| Synonyms: | USP4, UNP, Unph |
| Background: | The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | USP4: Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER. Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2. Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung. Expressed in the hippocampal neurons. Belongs to the peptidase C19 family. USP4 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.4.19.12; Oncoprotein; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: cytoplasm; cytosol; lysosome; nucleus; plasma membrane Molecular Function:adenosine receptor binding; identical protein binding; protein binding; ubiquitin-specific protease activity Biological Process: assembly of spliceosomal tri-snRNP; negative regulation of protein ubiquitination; protein deubiquitination; regulation of protein stability |
| NCBI Summary: | The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | Q13107 |
| NCBI GenInfo Identifier: | 116242839 |
| NCBI Gene ID: | 7375 |
| NCBI Accession: | Q13107.3 |
| UniProt Secondary Accession: | Q13107,O43452, O43453, Q08AK8, A8K6Y0, C9IY91, |
| UniProt Related Accession: | Q13107 |
| Molecular Weight: | 109kDa |
| NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase 4 |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 4 |
| NCBI Official Symbol: | USP4 |
| NCBI Official Synonym Symbols: | UNP; Unph |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 4 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 4 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme 4; Ubiquitin thioesterase 4; Ubiquitin-specific-processing protease 4; Ubiquitous nuclear protein homolog |
| UniProt Gene Name: | USP4 |
 | Western blot analysis of extracts of various cell lines, using USP4 antibody (CAB12242) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using USP4 Rabbit pAb (CAB12242) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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