Cell Biology Antibodies 6
Anti-USP33 Antibody (CAB16187)
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- SKU:
- CAB16187
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-USP33 Antibody |
| Antibody SKU: | CAB16187 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-400 of human USP33 (NP_963920.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-400 of human USP33 (NP_963920.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QVME VEED PQTI TTEE TMEE DKSQ SDVD FQSC ESCS NSDR AENE NGSR CFSE DNNE TTML IQDD ENNS EMSK DWQK EKMC NKIN KVNS EGEF DKDR DSIS ETVD LNNQ ETV |
| Gene ID: | 23032 |
| Uniprot: | Q8TEY7 |
| Cellular Location: | Cytoplasm, Golgi apparatus, centrosome, cytoskeleton, microtubule organizing center, perinuclear region |
| Calculated MW: | 93kDa/103kDa/106kDa |
| Observed MW: | 107KDa |
| Synonyms: | USP33, VDU1 |
| Background: | This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. |
| NCBI Summary: | This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012] |
| UniProt Code: | Q8TEY7 |
| NCBI GenInfo Identifier: | 42516567 |
| NCBI Gene ID: | 23032 |
| NCBI Accession: | NP_055832.3 |
| UniProt Secondary Accession: | Q8TEY7,A6QNM7, Q8TEY6, |
| UniProt Related Accession: | Q8TEY7 |
| Molecular Weight: | |
| NCBI Full Name: | ubiquitin carboxyl-terminal hydrolase 33 isoform 1 |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 33 |
| NCBI Official Symbol: | USP33 |
| NCBI Official Synonym Symbols: | VDU1 |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 33 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 33 |
| UniProt Synonym Protein Names: | Ubiquitin thiolesterase 33; Ubiquitin-specific-processing protease 33; Deubiquitinating enzyme 33; VHL-interacting deubiquitinating enzyme 1 |
| UniProt Gene Name: | USP33 |
| UniProt Entry Name: | UBP33_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using USP33 Polyclonal Antibody (CAB16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min. |
 | Western blot analysis of extracts of HepG2 cells, using USP33 Polyclonal Antibody (CAB16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Immunohistochemistry of paraffin-embedded human breast cancer using USP33 antibody (CAB16187) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat ovary using USP33 antibody (CAB16187) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using USP33 Polyclonal Antibody (CAB16187) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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