Cell Cycle Antibodies 1
Anti-USP3 Antibody (CAB15769)
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- SKU:
- CAB15769
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-USP3 Antibody |
| Antibody SKU: | CAB15769 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 321-520 of human USP3 (NP_006528.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 321-520 of human USP3 (NP_006528.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EVNC LICG TESR KFDP FLDL SLDI PSQF RSKR SKNQ ENGP VCSL RDCL RSFT DLEE LDET ELYM CHKC KKKQ KSTK KFWI QKLP KVLC LHLK RFHW TAYL RNKV DTYV EFPL RGLD MKCY LLEP ENSG PESC LYDL AAVV VHHG SGVG SGHY TAYA THEG RWFH FNDS TVTL TDEE TVVK AKAY ILFY VEHQ AKAG SDKL |
| Gene ID: | 9960 |
| Uniprot: | Q9Y6I4 |
| Cellular Location: | Nucleus |
| Calculated MW: | 53kDa/58kDa |
| Observed MW: | 67kDa |
| Synonyms: | USP3, SIH003, UBP |
| Background: |
| UniProt Protein Function: | USP3: Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin. Belongs to the peptidase C19 family. USP3 subfamily. |
| UniProt Protein Details: | Protein type:Protease; Ubiquitin-specific protease; EC 3.4.19.12; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 15q22.3 Cellular Component: nuclear chromatin Molecular Function:zinc ion binding; histone binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; chromatin binding Biological Process: proteasomal ubiquitin-dependent protein catabolic process; regulation of transcription, DNA-dependent; regulation of protein stability; mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; DNA repair; histone deubiquitination |
| UniProt Code: | Q9Y6I4 |
| NCBI GenInfo Identifier: | 205371844 |
| NCBI Gene ID: | 9960 |
| NCBI Accession: | Q9Y6I4.2 |
| UniProt Related Accession: | Q9Y6I4 |
| Molecular Weight: | Calculated: 53kDa; 58kDaObserved: 67kDa |
| NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase 3 |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 3 |
| NCBI Official Symbol: | USP3 |
| NCBI Official Synonym Symbols: | UBP; SIH003 |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 3 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 3 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme 3; Ubiquitin thioesterase 3; Ubiquitin-specific-processing protease 3 |
| UniProt Gene Name: | USP3 |
| UniProt Entry Name: | UBP3_HUMAN |
 | Western blot analysis of extracts of K-562 cells, using USP3 antibody (CAB15769) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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