Signal Transduction Antibodies 1
Anti-USP25 Antibody (CAB12588)
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- SKU:
- CAB12588
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-USP25 Antibody |
| Antibody SKU: | CAB12588 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human USP25 (NP_037528.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, HT-1080, U-251MG, HeLa, SKOV3, Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human USP25 (NP_037528.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTVE QNVL QQSA AQKH QQTF LNQL REIT GIND TQIL QQAL KDSN GNLE LAVA FLTA KNAK TPQQ EETT YYQT ALPG NDRY ISVG SQAD TNVI DLTG DDKD DLQR AIAL SLAE SNRA FRET GITD EEQA ISRV LEAS IAEN KACL KRTP TEVW RDSR NPYD RKRQ DKAP VGLK NVGN TCWF SAVI QSLF NLLE FRRL VLNY KPPS NAQD LPRN QKEH RNLP FMRE LRYL FA |
| Gene ID: | 29761 |
| Uniprot: | Q9UHP3 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 122kDa/125kDa/129kDa |
| Observed MW: | 130kDa |
| Synonyms: | USP25, USP21 |
| Background: |
| UniProt Protein Function: | USP25: Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Protease; Ubiquitin-specific protease; EC 3.4.19.12 Chromosomal Location of Human Ortholog: 21q11.2 Cellular Component: cytoplasm; nucleus Molecular Function:peptidase activity; protein binding; SUMO binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein modification process; proteolysis |
| NCBI Summary: | Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9UHP3 |
| NCBI GenInfo Identifier: | 302393833 |
| NCBI Gene ID: | 29761 |
| NCBI Accession: | Q9UHP3.4 |
| UniProt Secondary Accession: | Q9UHP3,Q6DHZ9, Q9H9W1, C0LSZ0, |
| UniProt Related Accession: | Q9UHP3 |
| Molecular Weight: | 1055 |
| NCBI Full Name: | Ubiquitin carboxyl-terminal hydrolase 25 |
| NCBI Synonym Full Names: | ubiquitin specific peptidase 25 |
| NCBI Official Symbol: | USP25 |
| NCBI Official Synonym Symbols: | USP21 |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase 25; USP on chromosome 21; ubiquitin thioesterase 25; deubiquitinating enzyme 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase 25 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme 25; USP on chromosome 21; Ubiquitin thioesterase 25; Ubiquitin-specific-processing protease 25 |
| UniProt Gene Name: | USP25 |
| UniProt Entry Name: | UBP25_HUMAN |
 | Western blot analysis of extracts of various cell lines, using USP25 antibody (CAB12588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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