Name: Anti-USP20 Antibody (CAB14643)
Brand: Cell Biology Antibodies 5
SKU: CAB14643
Availability: OutOfStock

Antibody Name:	Anti-USP20 Antibody
Antibody SKU:	CAB14643
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human USP20 (NP_006667.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human USP20 (NP_006667.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGDS RDLC PHLD SIGE VTKE DLLL KSKG TCQS CGVT GPNL WACL QVAC PYVG CGES FADH STIH AQAK KHNL TVNL TTFR LWCY ACEK EVFL EQRL AAPL LGSS SKFS EQDS PPPS HPLK AVPI AVAD EGES ESED DDLK PRGL TGMK NL
Gene ID:	10868
Uniprot:	Q9Y2K6
Cellular Location:	Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, perinuclear region
Calculated MW:	102kDa
Observed MW:	110kDa

Synonyms:

USP20, LSFR3A, VDU2, hVDU2

Background:

This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein.

UniProt Protein Function:	USP20: Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the peptidase C19 family. USP20/USP33 subfamily.
UniProt Protein Details:	Protein type:Ubiquitin-specific protease; EC 3.4.19.12; Ubiquitin conjugating system; Protease Chromosomal Location of Human Ortholog: 9q34.11 Cellular Component: centrosome Molecular Function:cysteine-type endopeptidase activity; G-protein-coupled receptor binding; protein binding Biological Process: protein deubiquitination; regulation of G-protein coupled receptor protein signaling pathway
NCBI Summary:	This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013]
UniProt Code:	Q9Y2K6
NCBI GenInfo Identifier:	116242837
NCBI Gene ID:	10868
NCBI Accession:	Q9Y2K6.2
UniProt Secondary Accession:	Q9Y2K6,Q541F1, Q8IXQ1, Q96LG5, Q9UQN8, Q9UQP0,
UniProt Related Accession:	Q9Y2K6
Molecular Weight:	102,003 Da
NCBI Full Name:	Ubiquitin carboxyl-terminal hydrolase 20
NCBI Synonym Full Names:	ubiquitin specific peptidase 20
NCBI Official Symbol:	USP20
NCBI Official Synonym Symbols:	VDU2; hVDU2; LSFR3A
NCBI Protein Information:	ubiquitin carboxyl-terminal hydrolase 20
UniProt Protein Name:	Ubiquitin carboxyl-terminal hydrolase 20
UniProt Synonym Protein Names:	Deubiquitinating enzyme 20; Ubiquitin thioesterase 20; Ubiquitin-specific-processing protease 20; VHL-interacting deubiquitinating enzyme 2; hVDU2
UniProt Gene Name:	USP20
UniProt Entry Name:	UBP20_HUMAN

Western blot analysis of extracts of HepG2 cells, using USP20 antibody (CAB14643) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

USP20 Antibody (PACO13123)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)