Anti-USF1 Antibody (CAB20903)

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SKU:
CAB20903
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Plant
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-USF1 Antibody (CAB20903)
Antibody SKU:CAB20903
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of Human USF1.
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, NIH/3T3, Jurkat, K-562, THP-1, Rat spleen
Immunogen:Recombinant protein of Human USF1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Nucleus
Calculated MW:27kDa/33kDa
Observed MW:36KDa
Synonyms:USF1, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, bHLHb11
Background:This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21.
Western blot analysis of extracts of Rat spleen, using USF1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of Rat spleen, using USF1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Western blot analysis of extracts of various cell lines, using USF1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Western blot analysis of extracts of various cell lines, using USF1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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