Antibody Name:	Anti-USF1 Antibody
Antibody SKU:	CAB13560
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human USF1 (NP_009053.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human USF1 (NP_009053.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKGQ QKTA ETEE GTVQ IQEG AVAT GEDP TSVA IASI QSAA TFPD PNVK YVFR TENG GQVM YRVI QVSE GQLD GQTE GTGA ISGY PATQ SMTQ AVIQ GAFT SDDA VDTE GTAA ETHY TYFP STAV GDGA GGTT SGST AAVV TTQG SEAL LGQA TPPG TGQF FVMM SPQE VLQG GSQR SIAP RTHP YSPK SEAP RTTR DEKR RAQH NEVE RRRR DKIN NWIV QLSK IIPD CSME STKS GQSK GGIL SKAC DYIQ ELRQ SNHR LSEE LQGL DQLQ LDND VLRQ QVED LKNK NLLL RAQL RHHG LEVV IKND SN
Gene ID:	7391
Uniprot:	P22415
Cellular Location:	Nucleus
Calculated MW:	27kDa/33kDa
Observed MW:	41kDa

Synonyms:

USF1, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, bHLHb11

Background:

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21.

UniProt Protein Function:	USF1: Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 1q22-q23 Cellular Component: nucleoplasm; transcription factor complex; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; protein homodimerization activity; sequence-specific DNA binding; protein heterodimerization activity; histone deacetylase binding; double-stranded DNA binding; bHLH transcription factor binding; protein kinase binding Biological Process: transcription from RNA polymerase II promoter; lipid homeostasis; glucose metabolic process; late viral mRNA transcription; glucose homeostasis; regulation of transcription from RNA polymerase II promoter; negative regulation of fibrinolysis; cellular response to insulin stimulus; regulation of transcription from RNA polymerase II promoter by glucose; response to hypoxia; regulation of transcription by carbon catabolites; positive regulation of transcription from RNA polymerase II promoter by glucose; positive regulation of transcription from RNA polymerase II promoter; response to UV Disease: Hyperlipidemia, Combined, 1
NCBI Summary:	This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21. [provided by RefSeq, Feb 2013]
UniProt Code:	P22415
NCBI GenInfo Identifier:	137170
NCBI Gene ID:	7391
NCBI Accession:	P22415.1
UniProt Related Accession:	P22415
Molecular Weight:
NCBI Full Name:	Upstream stimulatory factor 1
NCBI Synonym Full Names:	upstream transcription factor 1
NCBI Official Symbol:	USF1
NCBI Official Synonym Symbols:	UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11
NCBI Protein Information:	upstream stimulatory factor 1
UniProt Protein Name:	Upstream stimulatory factor 1
UniProt Synonym Protein Names:	Class B basic helix-loop-helix protein 11; bHLHb11; Major late transcription factor 1
Protein Family:	Upstream stimulatory factor
UniProt Gene Name:	USF1
UniProt Entry Name:	USF1_HUMAN

	Western blot analysis of extracts of mouse kidney, using USF1 antibody (CAB13560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human kidney cancer using USF1 antibody (CAB13560) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using USF1 antibody (CAB13560) at dilution of 1:200 (40x lens).