Autophagy Antibodies

Anti-ULK2 Antibody (CAB15244)

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SKU:
CAB15244
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy

Description

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Antibody Name:Anti-ULK2 Antibody
Antibody SKU:CAB15244
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ULK2 (NP_001136082.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, 293T, HepG2, K562, mouse brain, mouse kidney
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ULK2 (NP_001136082.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEVV GDFE YSKR DLVG HGAF AVVF RGRH RQKT DWEV AIKS INKK NLSK SQIL LGKE IKIL KELQ HENI VALY DVQE LPNS VFLV MEYC NGGD LADY LQAK
Gene ID:9706
Uniprot:Q8IYT8
Cellular Location:Cytoplasmic vesicle membrane, Peripheral membrane protein
Calculated MW:112kDa
Observed MW:113kDa
Synonyms:ULK2, ATG1B, Unc51.2
Background:This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified.
UniProt Protein Function:ULK2: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
UniProt Protein Details:

Protein type:Kinase, protein; Autophagy; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Other group; ULK family

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: pre-autophagosomal structure membrane

Molecular Function:protein binding; protein serine/threonine kinase activity

Biological Process: axon extension; protein amino acid autophosphorylation; regulation of autophagy; response to starvation

NCBI Summary:This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Dec 2008]
UniProt Code:Q8IYT8
NCBI GenInfo Identifier:296453001
NCBI Gene ID:9706
NCBI Accession:Q8IYT8.3
UniProt Secondary Accession:Q8IYT8,O75119, A8MY69,
UniProt Related Accession:Q8IYT8
Molecular Weight:112,694 Da
NCBI Full Name:Serine/threonine-protein kinase ULK2
NCBI Synonym Full Names:unc-51 like autophagy activating kinase 2
NCBI Official Symbol:ULK2  
NCBI Official Synonym Symbols:ATG1B; Unc51.2  
NCBI Protein Information:serine/threonine-protein kinase ULK2
UniProt Protein Name:Serine/threonine-protein kinase ULK2
UniProt Synonym Protein Names:Unc-51-like kinase 2
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:ULK2  
UniProt Entry Name:ULK2_HUMAN
Anti-ULK2 Antibody (CAB15244)Western blot analysis of extracts of various cell lines, using ULK2 antibody (CAB15244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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