Signal Transduction Antibodies 1
Anti-UFC1 Antibody (CAB13192)
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- SKU:
- CAB13192
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-UFC1 Antibody |
| Antibody SKU: | CAB13192 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human UFC1 (NP_057490.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, A-549, Mouse brain, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human UFC1 (NP_057490.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADE ATRR VVSE IPVL KTNA GPRD RELW VQRL KEEY QSLI RYVE NNKN ADND WFRL ESNK EGTR WFGK CWYI HDLL KYEF DIEF DIPI TYPT TAPE IAVP ELDG KTAK MYRG GKIC LTDH FKPL WARN VPKF GLAH LMAL GLGP WLAV EIPD LIQK GVIQ HKEK CNQ |
| Gene ID: | 51506 |
| Uniprot: | Q9Y3C8 |
| Cellular Location: | |
| Calculated MW: | 19kDa |
| Observed MW: | 19kDa |
| Synonyms: | UFC1, HSPC155 |
| Background: |
| UniProt Protein Function: | E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage. |
| NCBI Summary: | UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (UFM1; MIM 610553) (Komatsu et al., 2004 [PubMed 15071506]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9Y3C8 |
| NCBI GenInfo Identifier: | 156416000 |
| NCBI Gene ID: | 51506 |
| NCBI Accession: | NP_057490 |
| UniProt Secondary Accession: | Q9Y3C8,Q549X0, Q5VTX1, Q9BS96, Q9P009, A8K9R1, D3DVF9 |
| UniProt Related Accession: | Q9Y3C8 |
| Molecular Weight: | 21.6 kDa (187aa), confirmed by MALDI-TOF |
| NCBI Full Name: | ubiquitin-fold modifier-conjugating enzyme 1 |
| NCBI Synonym Full Names: | ubiquitin-fold modifier conjugating enzyme 1 |
| NCBI Official Symbol: | UFC1 |
| NCBI Official Synonym Symbols: | HSPC155 |
| NCBI Protein Information: | ubiquitin-fold modifier-conjugating enzyme 1 |
| UniProt Protein Name: | Ubiquitin-fold modifier-conjugating enzyme 1 |
| UniProt Gene Name: | UFC1 |
 | Western blot analysis of extracts of various cell lines, using UFC1 antibody (CAB13192) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using UFC1 Polyclonal Antibody (CAB13192) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using UFC1 Polyclonal Antibody (CAB13192) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using UFC1 Polyclonal Antibody (CAB13192) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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