Epigenetics & Nuclear Signaling Antibodies 1

Anti-UBR5 Antibody (CAB13816)

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SKU:
CAB13816
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-UBR5 Antibody
Antibody SKU:CAB13816
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 550-760 of human UBR5 (NP_056986.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, 293T, Jurkat, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 550-760 of human UBR5 (NP_056986.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SISA GIPK VGVL MESV WNMN DSCR FQLR SPES LKNM EKAS KTTE AKPE SKQE PVKT EMGP PPSP ASTC SDAS SIAS SASM PYKR RRST PAPK EEEK VNEE QWSL REVV FVED VKNV PVGK VLKV DGAY VAVK FPGT SSNT NCQN SSGP DADP SSLL QDCR LLRI DELQ VVKT GGTP KVPD CFQR TPKK LCIP EKTE ILAV NVDS KGVH AVL
Gene ID:51366
Uniprot:O95071
Cellular Location:Nucleus
Calculated MW:309kDa
Observed MW:300kDa
Synonyms:UBR5, DD5, EDD, EDD1, HYD
Background:This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.
UniProt Protein Function:UBR5: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Binds TOPBP1. Associates with CDK9 and TFIIS/TCEA1 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulates target gene transcription (e.g. gamma fibrinogen/FGG) by recruiting their promoters. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts directly with DYRK2. Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; EC 6.3.2.19; Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function:protein binding; ubiquitin binding; zinc ion binding; RNA binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; cell proliferation; protein polyubiquitination; progesterone receptor signaling pathway; positive regulation of protein import into nucleus, translocation; protein ubiquitination during ubiquitin-dependent protein catabolic process; DNA repair; response to DNA damage stimulus

NCBI Summary:This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. [provided by RefSeq, Jul 2008]
UniProt Code:O95071
NCBI GenInfo Identifier:9545980
NCBI Gene ID:51366
NCBI Accession:AAF88143.1
UniProt Secondary Accession:O95071,O94970, Q9NPL3, B2RP24, J3KMW7,
UniProt Related Accession:O95071
Molecular Weight:309,224 Da
NCBI Full Name:hyd protein
NCBI Synonym Full Names:ubiquitin protein ligase E3 component n-recognin 5
NCBI Official Symbol:UBR5  
NCBI Official Synonym Symbols:DD5; EDD; HYD; EDD1  
NCBI Protein Information:E3 ubiquitin-protein ligase UBR5; E3 identified by differential display; E3 ubiquitin protein ligase, HECT domain containing, 1; E3 ubiquitin-protein ligase, HECT domain-containing 1; hyperplastic discs protein homolog; progestin induced protein; progestin-induced protein
UniProt Protein Name:E3 ubiquitin-protein ligase UBR5
UniProt Synonym Protein Names:E3 ubiquitin-protein ligase, HECT domain-containing 1; Hyperplastic discs protein homolog; hHYD; Progestin-induced protein
Protein Family:Hydrolase
UniProt Gene Name:UBR5  
UniProt Entry Name:UBR5_HUMAN
Anti-UBR5 Antibody (CAB13816)Western blot analysis of extracts of various cell lines, using UBR5 antibody (CAB13816) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
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