Antibody Name:	Anti-Ubiquilin-2 Antibody
Antibody SKU:	CAB9568
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human UBQLN2 (NP_038472.2).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	B cells, NCI-H460, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human UBQLN2 (NP_038472.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAEN GESS GPPR PSRG PAAA QGSA AAPA EPKI IKVT VKTP KEKE EFAV PENS SVQQ FKEA ISKR FKSQ TDQL VLIF AGKI LKDQ DTLI QHGI HDGL TVHL VIKS QNRP QGQS TQPS NAAG TNTT SAST PRSN STPI STNS NPFG LGSL GG
Gene ID:	29978
Uniprot:	Q9UHD9
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, Membrane, Nucleus, autophagosome
Calculated MW:	65kDa
Observed MW:	70kDa

Synonyms:

UBQLN2, ALS15, CHAP1, DSK2, HRIHFB2157, N4BP4, PLIC2

Background:

This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases; and thus, are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein.

UniProt Protein Function:	UBQLN2: Increases the half-life of proteins destined to be degraded by the proteasome; may modulate proteasome-mediated protein degradation. Defects in UBQLN2 are the cause of amyotrophic lateral sclerosis type 15 with or without frontotemporal dementia (ALS15). A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. Patients with ALS15 may develop frontotemporal dementia.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: Xp11.21 Cellular Component: cytoplasm; plasma membrane Molecular Function:protein binding Biological Process: ER-associated protein catabolic process; regulation of macroautophagy Disease: Amyotrophic Lateral Sclerosis 15, With Or Without Frontotemporal Dementia
NCBI Summary:	This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases; and thus, are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein. [provided by RefSeq, Oct 2009]
UniProt Code:	Q9UHD9
NCBI GenInfo Identifier:	124056593
NCBI Gene ID:	29978
NCBI Accession:	Q9UHD9.2
UniProt Secondary Accession:	Q9UHD9,O94798, Q5D027, Q9H3W6, Q9HAZ4,
UniProt Related Accession:	Q9UHD9
Molecular Weight:	65,696 Da
NCBI Full Name:	Ubiquilin-2
NCBI Synonym Full Names:	ubiquilin 2
NCBI Official Symbol:	UBQLN2
NCBI Official Synonym Symbols:	DSK2; ALS15; CHAP1; N4BP4; PLIC2; HRIHFB2157
NCBI Protein Information:	ubiquilin-2
UniProt Protein Name:	Ubiquilin-2
UniProt Synonym Protein Names:	Chap1; DSK2 homolog; Protein linking IAP with cytoskeleton 2; PLIC-2; hPLIC-2; Ubiquitin-like product Chap1/Dsk2
Protein Family:	Ubiquilin
UniProt Gene Name:	UBQLN2
UniProt Entry Name:	UBQL2_HUMAN

	Western blot analysis of extracts of various cell lines, using UBQLN2 antibody (CAB9568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using UBQLN2 Polyclonal Antibody (CAB9568) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using UBQLN2 Polyclonal Antibody (CAB9568) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.