Name: Anti-UBE2N Antibody (CAB10543)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB10543
Availability: OutOfStock

Antibody Name:	Anti-UBE2N Antibody
Antibody SKU:	CAB10543
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2N (NP_003339.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, B cells, Mouse brain, Mouse spleen, Rat brain, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2N (NP_003339.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGL PRRI IKET QRLL AEPV PGIK AEPD ESNA RYFH VVIA GPQD SPFE GGTF KLEL FLPE EYPM AAPK VRFM TKIY HPNV DKLG RICL DILK DKWS PALQ IRTV LLSI QALL SAPN PDDP LAND VAEQ WKTN EAQA IETA RAWT RLYA MNNI
Gene ID:	7334
Uniprot:	P61088
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	17kDa
Observed MW:	17kDa

Synonyms:

UBE2N, HEL-S-71, UBC13, UBCHBEN, UbcH-ben, UbcH13

Background:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.

UniProt Protein Function:	The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.
NCBI Summary:	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]
UniProt Code:	P61088
NCBI GenInfo Identifier:	46577660
NCBI Gene ID:	7334
NCBI Accession:	P61088.1
UniProt Secondary Accession:	P61088,Q16781, Q53Y81,
UniProt Related Accession:	P61088
Molecular Weight:	17kDa
NCBI Full Name:	Ubiquitin-conjugating enzyme E2 N
NCBI Synonym Full Names:	ubiquitin conjugating enzyme E2 N
NCBI Official Symbol:	UBE2N
NCBI Official Synonym Symbols:	UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
NCBI Protein Information:	ubiquitin-conjugating enzyme E2 N
UniProt Protein Name:	Ubiquitin-conjugating enzyme E2 N
UniProt Synonym Protein Names:	Bendless-like ubiquitin-conjugating enzyme; E2 ubiquitin-conjugating enzyme N; Ubc13; UbcH13; Ubiquitin carrier protein N; Ubiquitin-protein ligase N
Protein Family:	Ubiquitin-conjugating enzyme
UniProt Gene Name:	UBE2N

Western blot analysis of extracts of various cell lines, using UBE2N antibody (CAB10543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Antibodies

Anti-Ube2N / Ubc13 Antibody (CAB9257)

UBE2N Antibody (PACO13035)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)