Signal Transduction Antibodies 2

Anti-UBE2D1 Antibody (CAB1951)

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SKU:
CAB1951
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-UBE2D1 Antibody
Antibody SKU:CAB1951
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D1 (NP_003329.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SW620, BT-474
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D1 (NP_003329.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MALK RIQK ELSD LQRD PPAH CSAG PVGD DLFH WQAT IMGP PDSA YQGG VFFL TVHF PTDY PFKP PKIA FTTK IYHP NINS NGSI CLDI LRSQ WSPA LTVS KVLL SICS LLCD PNPD DPLV PDIA QIYK SDKE KYNR HARE WTQK YAM
Gene ID:7321
Uniprot:P51668
Cellular Location:Cytoplasm
Calculated MW:16kDa
Observed MW:14kDa
Synonyms:UBE2D1, E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A
Background:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:UBE2D1: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP- induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4. Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol; ubiquitin ligase complex

Molecular Function:protein binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; transcription initiation from RNA polymerase II promoter; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; BMP signaling pathway; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein ubiquitination; transforming growth factor beta receptor signaling pathway; mitotic cell cycle spindle assembly checkpoint; toll-like receptor signaling pathway; innate immune response; gene expression; mitotic cell cycle; toll-like receptor 4 signaling pathway

NCBI Summary:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
UniProt Code:P51668
NCBI GenInfo Identifier:1717848
NCBI Gene ID:7321
NCBI Accession:P51668.1
UniProt Related Accession:P51668
Molecular Weight:
NCBI Full Name:Ubiquitin-conjugating enzyme E2 D1
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 D1
NCBI Official Symbol:UBE2D1  
NCBI Official Synonym Symbols:SFT; UBCH5; UBC4/5; UBCH5A; E2(17)KB1  
NCBI Protein Information:ubiquitin-conjugating enzyme E2 D1
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 D1
UniProt Synonym Protein Names:Stimulator of Fe transport; SFT; UBC4/5 homolog; UbcH5; Ubiquitin carrier protein D1; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-protein ligase D1
UniProt Gene Name:UBE2D1  
UniProt Entry Name:UB2D1_HUMAN
Anti-UBE2D1 Antibody (CAB1951)Western blot analysis of extracts of various cell lines, using UBE2D1 antibody (CAB1951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021).
Anti-UBE2D1 Antibody (CAB1951)Immunohistochemistry of paraffin-embedded rat brain using UBE2D1 antibody (CAB1951) at dilution of 1:100 (40x lens).
Anti-UBE2D1 Antibody (CAB1951)Immunohistochemistry of paraffin-embedded rat spleen using UBE2D1 antibody (CAB1951) at dilution of 1:100 (40x lens).
Anti-UBE2D1 Antibody (CAB1951)Immunohistochemistry of paraffin-embedded mouse spleen using UBE2D1 antibody (CAB1951) at dilution of 1:100 (40x lens).
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