Epigenetics & Nuclear Signaling Antibodies 5

Anti-U2AF65 Antibody (CAB4552)

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SKU:
CAB4552
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-U2AF65 Antibody
Antibody SKU:CAB4552
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human U2AF65
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HT-29, Mouse lung, Mouse brain, Rat brain
Immunogen:A synthesized peptide derived from human U2AF65
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:11338
Uniprot:P26368
Cellular Location:
Calculated MW:54kDa
Observed MW:65KDa
Synonyms:U2AF65
Background:U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008]
UniProt Protein Function:U2AF2: Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Belongs to the splicing factor SR family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA-binding; Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: nucleoplasm; spliceosome; nuclear speck

Molecular Function:protein binding; enzyme binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; positive regulation of RNA splicing

NCBI Summary:U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008]
UniProt Code:P26368
NCBI GenInfo Identifier:267188
NCBI Gene ID:11338
NCBI Accession:P26368.4
UniProt Secondary Accession:P26368,Q96HC5,
UniProt Related Accession:P26368
Molecular Weight:475
NCBI Full Name:Splicing factor U2AF 65 kDa subunit
NCBI Synonym Full Names:U2 small nuclear RNA auxiliary factor 2
NCBI Official Symbol:U2AF2  
NCBI Official Synonym Symbols:U2AF65  
NCBI Protein Information:splicing factor U2AF 65 kDa subunit; hU2AF65; U2 auxiliary factor 65 kDa subunit; U2 snRNP auxiliary factor large subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 small nuclear ribonucleoprotein auxiliary factor (65kD)
UniProt Protein Name:Splicing factor U2AF 65 kDa subunit
UniProt Synonym Protein Names:U2 auxiliary factor 65 kDa subunit; hU2AF(65); hU2AF65; U2 snRNP auxiliary factor large subunit
Protein Family:Splicing factor
UniProt Gene Name:U2AF2  
UniProt Entry Name:U2AF2_HUMAN
Anti-U2AF65 Antibody (CAB4552)Western blot - U2AF65 Rabbit mAb (CAB4552)
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