Name: Anti-TWF2 Antibody (CAB19897)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19897
Availability: OutOfStock

Antibody Name:	Anti-TWF2 Antibody [KO Validated]
Antibody SKU:	CAB19897
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human TWF2.

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human, Mouse
Positive Samples:	293T

Immunogen:	Recombinant protein of human TWF2.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	11344
Uniprot:	Q6IBS0
Cellular Location:	Cell projection, Cytoplasm, cytoskeleton, perinuclear region, stereocilium
Calculated MW:	39kDa
Observed MW:	40KDa

Anti-TWF2 Antibody [KO Validated] (CAB19897)

Western blot analysis of extracts from normal (control) and TWF2 knockout (KO) 293T cells, using TWF2 antibody (CAB19897) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:	TWF2, A6RP, A6r, MSTP011, PTK9L
Background:	The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein.

UniProt Protein Function:	A6r: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: stereocilium; cytoskeleton; growth cone; myofibril; lamellipodium; perinuclear region of cytoplasm; cytoplasm; filopodium Molecular Function:actin monomer binding; phosphatidylinositol-4,5-bisphosphate binding; protein kinase C binding; ATP binding Biological Process: cell projection organization and biogenesis; sequestering of actin monomers; negative regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; positive regulation of axon extension; regulation of microvillus length; barbed-end actin filament capping
NCBI Summary:	The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein. [provided by RefSeq, Jul 2008]
UniProt Code:	Q6IBS0
NCBI GenInfo Identifier:	94730596
NCBI Gene ID:	11344
NCBI Accession:	Q6IBS0.2
UniProt Secondary Accession:	Q6IBS0,Q9Y3F5,
UniProt Related Accession:	Q6IBS0
Molecular Weight:	349
NCBI Full Name:	Twinfilin-2
NCBI Synonym Full Names:	twinfilin actin-binding protein 2
NCBI Official Symbol:	TWF2
NCBI Official Synonym Symbols:	A6r; A6RP; PTK9L; MSTP011
NCBI Protein Information:	twinfilin-2; A6-related protein; twinfilin-1-like protein; twinfilin, actin-binding protein, homolog 2; protein tyrosine kinase 9-like (A6-related protein); PTK9L protein tyrosine kinase 9-like (A6-related protein)
UniProt Protein Name:	Twinfilin-2
UniProt Synonym Protein Names:	A6-related protein; hA6RP; Protein tyrosine kinase 9-like; Twinfilin-1-like protein
Protein Family:	Twinfilin
UniProt Gene Name:	TWF2
UniProt Entry Name:	TWF2_HUMAN