Name: Anti-TWF2 Antibody (CAB13642)
Brand: Cell Biology Antibodies 4
SKU: CAB13642
Availability: OutOfStock

Antibody Name:	Anti-TWF2 Antibody
Antibody SKU:	CAB13642
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human TWF2 (NP_009215.1).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human, Mouse
Positive Samples:	Jurkat, 293T, A-431, Mouse heart, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human TWF2 (NP_009215.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAHQ TGIH ATEE LKEF FAKA RAGS VRLI KVVI EDEQ LVLG ASQE PVGR WDQD YDRA VLPL LDAQ QPCY LLYR LDSQ NAQG FEWL FLAW SPDN SPVR LKML YAAT RATV KKEF GGGH IKDE LFGT VKDD LSFA GYQK HLSS CAAP APLT SAER ELQQ IRIN EVKT EISV ESKH QTLQ GLAF PLQP EAQR ALQQ LKQK MVNY IQMK LDLE RETI ELVH TEPT DVAQ LPSR VPRD AARY HFFL YKHT HEGD PLES VVFI YSMP GYKC SIKE RMLY SSCK SRLL DSVE QDFH LEIA KKIE IGDG AELT AEFL YDEV HPKQ HAFK QAFA KPKG PGGK RGHK RLIR GPGE NGDD S
Gene ID:	11344
Uniprot:	Q6IBS0
Cellular Location:	Cell projection, Cytoplasm, cytoskeleton, perinuclear region, stereocilium
Calculated MW:	39kDa
Observed MW:	40kDa

Synonyms:	TWF2, A6RP, A6r, MSTP011, PTK9L
Background:	The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein.

UniProt Protein Function:	A6r: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: stereocilium; cytoskeleton; growth cone; myofibril; lamellipodium; perinuclear region of cytoplasm; cytoplasm; filopodium Molecular Function:actin monomer binding; phosphatidylinositol-4,5-bisphosphate binding; protein kinase C binding; ATP binding Biological Process: cell projection organization and biogenesis; sequestering of actin monomers; negative regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; positive regulation of axon extension; regulation of microvillus length; barbed-end actin filament capping
NCBI Summary:	The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein. [provided by RefSeq, Jul 2008]
UniProt Code:	Q6IBS0
NCBI GenInfo Identifier:	94730596
NCBI Gene ID:	11344
NCBI Accession:	Q6IBS0.2
UniProt Secondary Accession:	Q6IBS0,Q9Y3F5,
UniProt Related Accession:	Q6IBS0
Molecular Weight:	349
NCBI Full Name:	Twinfilin-2
NCBI Synonym Full Names:	twinfilin actin-binding protein 2
NCBI Official Symbol:	TWF2
NCBI Official Synonym Symbols:	A6r; A6RP; PTK9L; MSTP011
NCBI Protein Information:	twinfilin-2; A6-related protein; twinfilin-1-like protein; twinfilin, actin-binding protein, homolog 2; protein tyrosine kinase 9-like (A6-related protein); PTK9L protein tyrosine kinase 9-like (A6-related protein)
UniProt Protein Name:	Twinfilin-2
UniProt Synonym Protein Names:	A6-related protein; hA6RP; Protein tyrosine kinase 9-like; Twinfilin-1-like protein
Protein Family:	Twinfilin
UniProt Gene Name:	TWF2
UniProt Entry Name:	TWF2_HUMAN

	Western blot analysis of extracts of various cell lines, using TWF2 antibody (CAB13642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug TWF2 antibody (CAB13642). Western blot was performed from the immunoprecipitate using TWF2 antibody (CAB13642) at a dilition of 1:1000.