KO Validated Antibodies 1

Anti-TTC11 / FIS1 Antibody (CAB5821)[KO Validated]

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SKU:
CAB5821
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-TTC11 / FIS1 Antibody [KO Validated]
Antibody SKU:CAB5821
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human TTC11 / FIS1 (NP_057152.2).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human TTC11 / FIS1 (NP_057152.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEAV LNEL VSVE DLLK FEKK FQSE KAAG SVSK STQF EYAW CLVR SKYN DDIR KGIV LLEE LLPK GSKE EQRD YVFY LAVG NYRL KEYE KALK YVRG LLQT EPQN NQAK ELER LIDK AMKK DG
Gene ID:51024
Uniprot:Q9Y3D6
Cellular Location:Mitochondrion outer membrane, Peroxisome membrane, Single-pass membrane protein
Calculated MW:16kDa
Observed MW:17KDa
Synonyms:CGI-135, TTC11, FIS1, TTC11 / FIS1, fission
Background:The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM, Mar 2008]
UniProt Protein Function:FIS1: Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission. Belongs to the FIS1 family.
UniProt Protein Details:

Protein type:Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: integral to mitochondrial outer membrane; integral to peroxisomal membrane; membrane; mitochondrion; peroxisome; protein complex

Molecular Function:protein binding; receptor binding

Biological Process: elevation of cytosolic calcium ion concentration; elevation of mitochondrial calcium ion concentration; mitochondrial fission; mitochondrial fragmentation during apoptosis; mitochondrial fusion; mitochondrion degradation; peroxisome fission; positive regulation of caspase activity; protein homooligomerization; protein targeting to mitochondrion; reduction of endoplasmic reticulum calcium ion concentration; release of cytochrome c from mitochondria

NCBI Summary:The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9Y3D6
NCBI GenInfo Identifier:33112470
NCBI Gene ID:51024
NCBI Accession:Q9Y3D6.2
UniProt Secondary Accession:Q9Y3D6,Q9BTP3,
UniProt Related Accession:Q9Y3D6
Molecular Weight:16,938 Da
NCBI Full Name:Mitochondrial fission 1 protein
NCBI Synonym Full Names:fission, mitochondrial 1
NCBI Official Symbol:FIS1  
NCBI Official Synonym Symbols:TTC11; CGI-135  
NCBI Protein Information:mitochondrial fission 1 protein
UniProt Protein Name:Mitochondrial fission 1 protein
UniProt Synonym Protein Names:FIS1 homolog; hFis1; Tetratricopeptide repeat protein 11; TPR repeat protein 11
Protein Family:Mitochondria fission 1 protein
UniProt Gene Name:FIS1  
UniProt Entry Name:FIS1_HUMAN
Anti-TTC11 / FIS1 Antibody [KO Validated] (CAB5821)Western blot analysis of extracts of 293T cells, using TTC11 / FIS1 Rabbit pAb (CAB5821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-TTC11 / FIS1 Antibody [KO Validated] (CAB5821)Western blot analysis of extracts from normal (control) and TTC11 / FIS1 Rabbit pAb knockout (KO) HeLa cells, using TTC11 / FIS1 Rabbit pAb antibody (CAB5821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-TTC11 / FIS1 Antibody [KO Validated] (CAB5821)Immunohistochemistry of paraffin-embedded rat heart using TTC11 / FIS1 Antibody (CAB5821) at dilution of 1:100 (40x lens).
Anti-TTC11 / FIS1 Antibody [KO Validated] (CAB5821)Immunohistochemistry of paraffin-embedded rat heart using TTC11 / FIS1 Antibody (CAB5821) at dilution of 1:100 (40x lens).
Anti-TTC11 / FIS1 Antibody [KO Validated] (CAB5821)Immunohistochemistry of paraffin-embedded mouse heart using TTC11 / FIS1 Antibody (CAB5821) at dilution of 1:100 (40x lens).
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