Anti-TSFM Antibody (CAB20936)

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SKU:
CAB20936
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-TSFM Antibody (CAB20936)
Antibody SKU:CAB20936
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence, Immunoprecipitation
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of Human TSFM.
Application:Western blotting, Immunofluorescence, Immunoprecipitation
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HepG2, HeLa, RAW264.7, Mouse heart, Rat liver
Immunogen:Recombinant protein of Human TSFM.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Mitochondrion
Calculated MW:18kDa/23kDa/35kDa/37kDa
Observed MW:35kDa
Synonyms:TSFM, EFTS, EFTSMT
Background:This gene encodes a mitochondrial translation elongation factor. The encoded protein is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in this gene are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants.
Immunofluorescence analysis of HeLa cells using TSFM Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of HeLa cells using TSFM Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug TSFM antibody . Western blot was performed from the immunoprecipitate using TSFM antibody at a dilition of 1:500.Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug TSFM antibody . Western blot was performed from the immunoprecipitate using TSFM antibody at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using TSFM antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Western blot analysis of extracts of various cell lines, using TSFM antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
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