Name: Anti-TSC22D3 Antibody (CAB19911)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19911
Availability: OutOfStock

Antibody Name:	Anti-TSC22D3 Antibody [KO Validated]
Antibody SKU:	CAB19911
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human TSC22D3.

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant protein of human TSC22D3.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	1831
Uniprot:	Q99576
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	13kDa/14kDa/22kDa
Observed MW:	16KDa

Anti-TSC22D3 Antibody [KO Validated] (CAB19911)

Western blot analysis of extracts from normal (control) and TSC22D3 knockout (KO) 293T cells, using TSC22D3 antibody (CAB19911) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Synonyms:

TSC22D3, DIP, DSIPI, GILZ, TSC-22R

Background:

This gene encodes the anti-inflammatory protein glucocorticoid (GC)-induced leucine zipper. Expression of this gene stimulated by glucocorticoids and interleukin 10 and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid. This protein has also been shown to inhibit pro-inflammatory molecules including nuclear factor ?B. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	GILZ: Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities. Belongs to the TSC-22/Dip/Bun family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: Xq22.3 Cellular Component: cytosol Biological Process: regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes the anti-inflammatory protein glucocorticoid (GC)-induced leucine zipper. Expression of this gene stimulated by glucocorticoids and interleukin 10 and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid. This protein has also been shown to inhibit pro-inflammatory molecules including nuclear factor κB. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
UniProt Code:	Q99576
NCBI GenInfo Identifier:	14195584
NCBI Gene ID:	1831
NCBI Accession:	Q99576.2
UniProt Secondary Accession:	Q99576,Q5H9S3, Q5JRI9, Q6FIH6, Q8NAI1, Q8WVB9, Q9UBN5 Q9UG13,
UniProt Related Accession:	Q99576
Molecular Weight:	13,286 Da
NCBI Full Name:	TSC22 domain family protein 3
NCBI Synonym Full Names:	TSC22 domain family member 3
NCBI Official Symbol:	TSC22D3
NCBI Official Synonym Symbols:	DIP; GILZ; DSIPI; TSC-22R
NCBI Protein Information:	TSC22 domain family protein 3
UniProt Protein Name:	TSC22 domain family protein 3
UniProt Synonym Protein Names:	DSIP-immunoreactive peptide; Protein DIP; hDIP; Delta sleep-inducing peptide immunoreactor; Glucocorticoid-induced leucine zipper protein; GILZ; TSC-22-like protein; TSC-22-related protein; TSC-22R
Protein Family:	TSC22 domain family protein
UniProt Gene Name:	TSC22D3
UniProt Entry Name:	T22D3_HUMAN

Antibodies	ELISA Kits
Anti-TSC22D3 Antibody (CAB6779)	Human GILZ (Glucocorticoid-induced leucine zipper protein) ELISA Kit (HUFI04128)
TSC22D3 Antibody (PACO12948)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)