Cell Biology Antibodies 5

Anti-TRPC6 Antibody (CAB15107)

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SKU:
CAB15107
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TRPC6 Antibody
Antibody SKU:CAB15107
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 732-931 of human TRPC6 (NP_004612.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SGC996, Mouse lung, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 732-931 of human TRPC6 (NP_004612.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QEIE DDAD VEWK FARA KLWF SYFE EGRT LPVP FNLV PSPK SLFY LLLK LKKW ISEL FQGH KKGF QEDA EMNK INEE KKLG ILGS HEDL SKLS LDKK QVGH NKQP SIRS SEDF HLNS FNNP PRQY QKIM KRLI KRYV LQAQ IDKE SDEV NEGE LKEI KQDI SSLR YELL EEKS QNTE DLAE LIRE LGEK LSME PNQE ETNR
Gene ID:7225
Uniprot:Q9Y210
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:93kDa/100kDa/106kDa
Observed MW:110kDa
Synonyms:TRPC6, FSGS2, TRP6
Background:The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2).
UniProt Protein Function:TRPC6: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. Interacts with MX1 and RNF24. Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm. Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Channel, cation; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q22.1

Cellular Component: plasma membrane; integral to membrane

Molecular Function:protein binding; store-operated calcium channel activity

Biological Process: positive regulation of ion transmembrane transporter activity; platelet activation; axon guidance; elevation of cytosolic calcium ion concentration; blood coagulation; positive regulation of calcium ion transport; transmembrane transport; cation transport; aging

Disease: Focal Segmental Glomerulosclerosis 2

NCBI Summary:The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). [provided by RefSeq, Mar 2009]
UniProt Code:Q9Y210
NCBI GenInfo Identifier:6686048
NCBI Gene ID:7225
NCBI Accession:Q9Y210.1
UniProt Secondary Accession:Q9Y210,Q52M59, Q9HCW3, Q9NQA8, Q9NQA9,
UniProt Related Accession:Q9Y210
Molecular Weight:100,106 Da
NCBI Full Name:Short transient receptor potential channel 6
NCBI Synonym Full Names:transient receptor potential cation channel, subfamily C, member 6
NCBI Official Symbol:TRPC6  
NCBI Official Synonym Symbols:TRP6; FSGS2  
NCBI Protein Information:short transient receptor potential channel 6; TRP-6; transient receptor protein 6; focal segmental glomerulosclerosis 2
UniProt Protein Name:Short transient receptor potential channel 6
UniProt Synonym Protein Names:Transient receptor protein 6; TRP-6
Protein Family:Short transient receptor potential channel
UniProt Gene Name:TRPC6  
UniProt Entry Name:TRPC6_HUMAN
Anti-TRPC6 Antibody (CAB15107)Western blot analysis of extracts of various cell lines, using TRPC6 antibody (CAB15107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-TRPC6 Antibody (CAB15107)Immunofluorescence analysis of HeLa cells using TRPC6 antibody (CAB15107) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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