ChIP Antibodies

Anti-TRMT1 Antibody (CAB7116)

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SKU:
CAB7116
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-TRMT1 Antibody
Antibody SKU:CAB7116
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 460-659 of human TRMT1 (NP_060192.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:MCF7, HL-60, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 460-659 of human TRMT1 (NP_060192.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PSLL QLRS ALLH ADFR VSLS HACK NAVK TDAP ASAL WDIM RCWE KECP VKRE RLSE TSPA FRIL SVEP RLQA NFTI REDA NPSS RQRG LKRF QANP EANW GPRP RARP GGKA ADEA MEER RRLL QNKR KEPP EDVA QRAA RLKT FPCK RFKE GTCQ RGDQ CCYS HSPP TPRV SADA APDC PETS NQTP PGPG AAAG PGID
Gene ID:55621
Uniprot:Q9NXH9
Cellular Location:
Calculated MW:69kDa/72kDa
Observed MW:90kDa
Synonyms:TRMT1, TRM1
Background:
UniProt Protein Function:TRMT1: Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Methyltransferase; EC 2.1.1.216

Chromosomal Location of Human Ortholog: 19p13.2

Molecular Function:metal ion binding; tRNA (guanine-N2-)-methyltransferase activity; tRNA binding

Biological Process: tRNA methylation

UniProt Code:Q9NXH9
NCBI GenInfo Identifier:12643821
NCBI Gene ID:55621
NCBI Accession:Q9NXH9.1
UniProt Secondary Accession:Q9NXH9,O76103, Q548Y5, Q8WVA6,
UniProt Related Accession:Q9NXH9
Molecular Weight:659
NCBI Full Name:tRNA (guanine(26)-N(2))-dimethyltransferase
NCBI Synonym Full Names:tRNA methyltransferase 1 homolog (S. cerevisiae)
NCBI Official Symbol:TRMT1  
NCBI Official Synonym Symbols:TRM1  
NCBI Protein Information:tRNA (guanine(26)-N(2))-dimethyltransferase; tRNA (guanine(26)-N(2))-dimethyltransferase; tRNA(m(2,2)G26)dimethyltransferase; TRM1 tRNA methyltransferase 1 homolog; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA 2,2-dimethylguanosine-26 methyltransferase; N(2),N(2)-dimethylguanosine tRNA methyltransferase
UniProt Protein Name:tRNA (guanine(26)-N(2))-dimethyltransferase
UniProt Synonym Protein Names:tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase
Protein Family:TRMT1-like protein
UniProt Gene Name:TRMT1  
UniProt Entry Name:TRM1_HUMAN
Anti-TRMT1 Antibody (CAB7116)Western blot analysis of extracts of various cell lines, using TRMT1 antibody (CAB7116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-TRMT1 Antibody (CAB7116)Immunohistochemistry of paraffin-embedded human lung cancer using TRMT1 antibody (CAB7116) at dilution of 1:100 (40x lens).
Anti-TRMT1 Antibody (CAB7116)Immunohistochemistry of paraffin-embedded human stomach using TRMT1 antibody (CAB7116) at dilution of 1:100 (40x lens).
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