Description
Antibody Name: | Anti-TRIM33 Antibody (CAB20811) |
Antibody SKU: | CAB20811 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 950-1090 of human TRIM33 (NP_056990.3). |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Rat |
Positive Samples: | MCF7, K-562, Rat testis |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 950-1090 of human TRIM33 (NP_056990.3). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | KKGK TAQG LSPV DQRK CERL LLYL YCHE LSIE FQEP VPAS IPNY YKII KKPM DLST VKKK LQKK HSQH YQIP DDFV ADVR LIFK NCER FNEM MKVV QVYA DTQE INLK ADSE VAQA GKAV ALYF EDKL TEIY SDRT FAPL P |
Cellular Location: | |
Calculated MW: | 122kDa |
Observed MW: | 150KDa |
Synonyms: | ECTO, PTC7, RFG7, TF1G, TIF1G, TIFGAMMA, TIF1GAMMA |
Background: | The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. |
Western blot analysis of extracts of various cell lines, using TRIM33 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s. |