Immunology Antibodies 1

Anti-TRIM25 Antibody (CAB12938)

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SKU:
CAB12938
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-TRIM25 Antibody
Antibody SKU:CAB12938
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM25 (NP_005073.2).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HepG2, A-431, Mouse uterus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM25 (NP_005073.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SPNA QVAC DHCL KEAA VKTC LVCM ASFC QEHL QPHF DSPA FQDH PLQP PVRD LLRR KCSQ HNRL REFF CPEH SECI CHIC LVEH KTCS PASL SQAS ADLE ATLR HKLT VMYS QING ASRA LDDV RNRQ QDVR MTAN RKVE QLQQ EYTE MKAL LDAS ETTS TRKI KEEE KRVN SKFD TIYQ ILLK KKSE IQTL KEEI EQSL TKRD EFEF LEKA SKLR GIST KPVY IPEV ELNH KLIK GIHQ STID LKNE LKQC IGRL QEPT PSSG DPGE HDPA STHK STRP VKKV SKEE KKSK KPPP VPAL P
Gene ID:7706
Uniprot:Q14258
Cellular Location:Cytoplasm
Calculated MW:70kDa
Observed MW:68kDa
Synonyms:TRIM25, EFP, RNF147, Z147, ZNF147
Background:The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
UniProt Protein Function:TRIM25: a ubiquitous protein that mediates estrogen action in various target organs. Contains one RING-type zinc finger and one SPRY domain.
UniProt Protein Details:

Protein type:EC 6.3.2.19; Ubiquitin conjugating system; Transcription factor; Ubiquitin ligase; EC 6.3.2.n3; Ligase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function:protein binding; acid-amino acid ligase activity; zinc ion binding; transcription factor activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; protein ubiquitination; regulation of virion penetration into host cell; activation of NF-kappaB transcription factor; response to vitamin D; response to estrogen stimulus; innate immune response; positive regulation of transcription factor activity; defense response to virus; negative regulation of interferon type I production

NCBI Summary:The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q14258
NCBI GenInfo Identifier:313104033
NCBI Gene ID:7706
NCBI Accession:Q14258.2
UniProt Related Accession:Q14258
Molecular Weight:630
NCBI Full Name:E3 ubiquitin/ISG15 ligase TRIM25
NCBI Synonym Full Names:tripartite motif containing 25
NCBI Official Symbol:TRIM25  
NCBI Official Synonym Symbols:EFP; Z147; RNF147; ZNF147  
NCBI Protein Information:E3 ubiquitin/ISG15 ligase TRIM25; RING finger protein 147; zinc finger protein-147; tripartite motif-containing 25; tripartite motif protein TRIM25; estrogen-responsive finger protein; tripartite motif-containing protein 25; ubiquitin/ISG15-conjugating enzyme TRIM25; zinc finger protein 147 (estrogen-responsive finger protein)
UniProt Protein Name:E3 ubiquitin/ISG15 ligase TRIM25
UniProt Synonym Protein Names:Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147
Protein Family:E3 ubiquitin/ISG15 ligase
UniProt Gene Name:TRIM25  
UniProt Entry Name:TRI25_HUMAN
Anti-TRIM25 Antibody (CAB12938)Western blot analysis of extracts of various cell lines, using TRIM25 antibody (CAB12938) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-TRIM25 Antibody (CAB12938)Immunofluorescence analysis of L929 cells using TRIM25 Rabbit pAb (CAB12938) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-TRIM25 Antibody (CAB12938)Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug TRIM25 antibody (CAB12938). Western blot was performed from the immunoprecipitate using TRIM25 antibody (CAB12938) at a dilition of 1:1000.
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