Name: Anti-TREX1 Antibody (CAB6778)
Brand: Cell Biology Antibodies 10
SKU: CAB6778
Availability: OutOfStock

Antibody Name:	Anti-TREX1 Antibody
Antibody SKU:	CAB6778
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TREX1 (NP_057465.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, HeLa, BT-474, SW480, Raji, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TREX1 (NP_057465.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGPG ARRQ GRIV QGRP EMCF CPPP TPLP PLRI LTLG THTP TPCS SPGS AAGT YPTM GSQA LPPG PMQT LIFF DMEA TGLP FSQP KVTE LCLL AVHR CALE SPPT SQGP PPTV PPPP RVVD KLSL CVAP GKAC SPAA SEIT GLST AVLA AHGR QCFD DNLA NLLL AFLR RQPQ PWCL VAHN GDRY DFPL LQAE LAML GLTS ALDG AFCV DSIT ALKA LERA SSPS EHGP RKSY SLGS IYTR LYGQ SPPD SHTA EGDV LALL SICQ WRPQ AL
Gene ID:	11277
Uniprot:	Q9NSU2
Cellular Location:	Cytoplasm, Endoplasmic reticulum membrane, Nucleus, Peripheral membrane protein, cytosol
Calculated MW:	32kDa/33kDa/38kDa
Observed MW:	37kDa

Synonyms:	TREX1, AGS1, CRV, DRN3, HERNS
Background:	This gene encodes a nuclear protein with 3' exonuclease activity. The encoded protein may play a role in DNA repair and serve as a proofreading function for DNA polymerase. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, Cree encephalitis, and other diseases of the immune system. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	TREX1: the major 3' DNA exonuclease in mammalian cells. Normally associates with the endoplasmic reticulum (ER). Translocates to the nucleus at S phase after DNA damage by gamma-irradiation or hydroxyurea. Trex1-deficient cells show defective G1/S transition, with single-stranded DNA molecules persisting after S phase and accumulating in the ER. Degrades ssDNA. Prevents chronic checkpoint activation and inappropriate immune activation. Mutations cause Aicardi-Goutieres syndrome, an autoimmune disorder. Trex1a(-/-) mice have autoinflammatory responses. The gene for this protein is either identical to or adjacent to that of ATRIP. Some of the mRNAs that encode TREX1 also encode ATRIP in another reading frame. Three alternatively spliced human isoforms have been reported.
UniProt Protein Details:	Protein type:Cell cycle regulation; DNA repair, damage; Deoxyribonuclease; EC 3.1.11.2; DNA-binding Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: endoplasmic reticulum membrane; nucleolus; nuclear envelope; nucleus; cytosol Molecular Function:protein homodimerization activity; metal ion binding; double-stranded DNA binding; 3'-5'-exodeoxyribonuclease activity; exodeoxyribonuclease III activity; MutLalpha complex binding; adenyl deoxyribonucleotide binding; 3'-5' exonuclease activity; MutSalpha complex binding; single-stranded DNA binding Biological Process: regulation of interferon type I production; mismatch repair; positive regulation of interferon type I production; innate immune response; DNA replication; DNA repair; DNA catabolic process, exonucleolytic; DNA metabolic process; DNA recombination Disease: Chilblain Lupus 1; Vasculopathy, Retinal, With Cerebral Leukodystrophy; Aicardi-goutieres Syndrome 1; Systemic Lupus Erythematosus
NCBI Summary:	This gene encodes a nuclear protein with 3' exonuclease activity. The encoded protein may play a role in DNA repair and serve as a proofreading function for DNA polymerase. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, Cree encephalitis, and other diseases of the immune system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]
UniProt Code:	Q9NSU2
NCBI GenInfo Identifier:	47606216
NCBI Gene ID:	11277
NCBI Accession:	Q9NSU2.1
UniProt Secondary Accession:	Q9NSU2,Q8TEU2, Q9BPW1, Q9Y4X2, B2RCN9,
UniProt Related Accession:	Q9NSU2
Molecular Weight:	369
NCBI Full Name:	Three-prime repair exonuclease 1
NCBI Synonym Full Names:	three prime repair exonuclease 1
NCBI Official Symbol:	TREX1
NCBI Official Synonym Symbols:	CRV; AGS1; DRN3; HERNS
NCBI Protein Information:	three-prime repair exonuclease 1; DNase III; deoxyribonuclease III; 3' repair exonuclease 1; 3'-5' exonuclease TREX1
UniProt Protein Name:	Three-prime repair exonuclease 1
UniProt Synonym Protein Names:	3'-5' exonuclease TREX1; DNase III
Protein Family:	Three-prime repair exonuclease
UniProt Gene Name:	TREX1
UniProt Entry Name:	TREX1_HUMAN

	Western blot analysis of extracts of various cell lines, using TREX1 antibody (CAB6778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of L929 cells using TREX1 antibody (CAB6778) at dilution of 1:100. Blue: DAPI for nuclear staining.