Cell Biology Antibodies 8
Anti-TRAP1 Antibody (CAB2748)
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- SKU:
- CAB2748
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TRAP1 Antibody |
| Antibody SKU: | CAB2748 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human TRAP1 |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW620, MCF7, Jurkat, BxPC-3, Mouse kidney, Mouse skeletal muscle, Mouse heart, Mouse brain |
| Immunogen: | Recombinant protein of human TRAP1 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 10131 |
| Uniprot: | Q12931 |
| Cellular Location: | Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix |
| Calculated MW: | 74kDa/80kDa |
| Observed MW: | 80kDa |
| Synonyms: | TRAP1, HSP 75, HSP75, HSP90L, TRAP-1 |
| Background: | This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | HSP75: Chaperone that expresses an ATPase activity. Belongs to the heat shock protein 90 family. |
| UniProt Protein Details: | Protein type:Chaperone; Heat shock protein Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: nucleoplasm; membrane; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; mitochondrial intermembrane space; lipid particle Molecular Function:protein binding; unfolded protein binding; protein kinase binding; tumor necrosis factor receptor binding; ATP binding Biological Process: response to stress |
| NCBI Summary: | This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
| UniProt Code: | Q12931 |
| NCBI GenInfo Identifier: | 67477458 |
| NCBI Gene ID: | 10131 |
| NCBI Accession: | Q12931.3 |
| UniProt Related Accession: | Q12931 |
| Molecular Weight: | 75kDa |
| NCBI Full Name: | Heat shock protein 75 kDa, mitochondrial |
| NCBI Synonym Full Names: | TNF receptor associated protein 1 |
| NCBI Official Symbol: | TRAP1 |
| NCBI Official Synonym Symbols: | HSP75; HSP 75; HSP90L; TRAP-1 |
| NCBI Protein Information: | heat shock protein 75 kDa, mitochondrial |
| UniProt Protein Name: | Heat shock protein 75 kDa, mitochondrial |
| UniProt Synonym Protein Names: | TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1 |
| UniProt Gene Name: | TRAP1 |
| UniProt Entry Name: | TRAP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TRAP1 antibody (CAB2748) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat heart using TRAP1 antibody (CAB2748) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using TRAP1 antibody (CAB2748) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using TRAP1 antibody (CAB2748) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using TRAP1 Polyclonal Antibody (CAB2748) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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