Cell Biology Antibodies 1
Anti-TPMT Antibody (CAB1017)
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- SKU:
- CAB1017
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TPMT Antibody |
| Antibody SKU: | CAB1017 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human TPMT (NP_000358.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human |
| Positive Samples: | K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human TPMT (NP_000358.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDGT RTSL DIEE YSDT EVQK NQVL TLEE WQDK WVNG KTAF HQEQ GHQL LKKH LDTF LKGK SGLR VFFP LCGK AVEM KWFA DRGH SVVG VEIS ELGI QEFF TEQN LSYS EEPI TEIP GTKV FKSS SGNI SLYC CSIF DLPR TNIG KFDM IWDR GALV AINP GDRK CYAD TMFS LLGK KFQY LLCV LSYD PTKH PGPP FYVP HAEI ERLF GKIC NIRC LEKV DAFE ERHK SWGI DCLF EKLY LLTE K |
| Gene ID: | 7172 |
| Uniprot: | P51580 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 28kDa |
| Observed MW: | 28kDa |
| Synonyms: | TPMT, TPMTD |
| Background: | This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. |
| UniProt Protein Function: | TPMT: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. Defects in TPMT are the cause of thiopurine S- methyltransferase deficiency (TPMT deficiency). TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. Belongs to the methyltransferase superfamily. TPMT family. |
| UniProt Protein Details: | Protein type:Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.1.1.67; Methyltransferase Chromosomal Location of Human Ortholog: 6p22.3 Cellular Component: cytosol Molecular Function:thiopurine S-methyltransferase activity Biological Process: methylation; xenobiotic metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process Disease: Thiopurine S-methyltransferase Deficiency |
| NCBI Summary: | This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014] |
| UniProt Code: | P51580 |
| NCBI GenInfo Identifier: | 1730006 |
| NCBI Gene ID: | 7172 |
| NCBI Accession: | P51580.1 |
| UniProt Secondary Accession: | P51580,O14806, O15423, O15424, O15425, O15426, O15515 O15548, O43213, Q5VUK6, Q9UBE6, Q9UBT8, |
| UniProt Related Accession: | P51580 |
| Molecular Weight: | 245 |
| NCBI Full Name: | Thiopurine S-methyltransferase |
| NCBI Synonym Full Names: | thiopurine S-methyltransferase |
| NCBI Official Symbol: | TPMT |
| NCBI Protein Information: | thiopurine S-methyltransferase; S-adenosyl-L-methionine:thiopurine S-methyltransferase |
| UniProt Protein Name: | Thiopurine S-methyltransferase |
| UniProt Synonym Protein Names: | Thiopurine methyltransferase |
| UniProt Gene Name: | TPMT |
| UniProt Entry Name: | TPMT_HUMAN |
 | Western blot analysis of extracts of K562 cells, using TPMT antibody (CAB1017) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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