Metabolism Antibodies 2
Anti-TPI1 Antibody (CAB2579)
- SKU:
- CAB2579
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-TPI1 Antibody |
| Antibody SKU: | CAB2579 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human TPI1 (NP_000356.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 22Rv1, BT-474, HeLa, Jurkat, U-251MG, HepG2, Mouse kidney, Mouse brain, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human TPI1 (NP_000356.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAPS RKFF VGGN WKMN GRKQ SLGE LIGT LNAA KVPA DTEV VCAP PTAY IDFA RQKL DPKI AVAA QNCY KVTN GAFT GEIS PGMI KDCG ATWV VLGH SERR HVFG ESDE LIGQ KVAH ALAE GLGV IACI GEKL DERE AGIT EKVV FEQT KVIA DNVK DWSK VVLA YEPV WAIG TGKT ATPQ QAQE VHEK LRGW LKSN VSDA VAQS TRII YGGS VTGA TCKE LASQ PDVD GFLV GGAS LKPE FVDI INAK Q |
| Gene ID: | 7167 |
| Uniprot: | P60174 |
| Cellular Location: | |
| Calculated MW: | 17kDa/26kDa/30kDa |
| Observed MW: | 27kDa |
| Synonyms: | TPI1, HEL-S-49, TIM, TPI, TPID |
| Background: | This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter. |
| UniProt Protein Details: | Protein type:EC 5.3.1.1; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase; Carbohydrate Metabolism - inositol phosphate; Carbohydrate Metabolism - fructose and mannose Chromosomal Location of Human Ortholog: 12p13 Cellular Component: extracellular space; cytosol; nucleus Molecular Function:protein binding; triose-phosphate isomerase activity Biological Process: pentose-phosphate shunt; glycolysis; multicellular organismal development; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis Disease: Triosephosphate Isomerase Deficiency |
| NCBI Summary: | This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
| UniProt Code: | P60174 |
| NCBI GenInfo Identifier: | 353526311 |
| NCBI Gene ID: | 7167 |
| NCBI Accession: | P60174.3 |
| UniProt Secondary Accession: | P60174,P00938, Q6FHP9, Q6IS07, Q8WWD0, Q96AG5, B7Z5D8 D3DUS9, |
| UniProt Related Accession: | P60174 |
| Molecular Weight: | 249 |
| NCBI Full Name: | Triosephosphate isomerase |
| NCBI Synonym Full Names: | triosephosphate isomerase 1 |
| NCBI Official Symbol: | TPI1 |
| NCBI Official Synonym Symbols: | TIM; TPI; TPID; HEL-S-49 |
| NCBI Protein Information: | triosephosphate isomerase; triose-phosphate isomerase; epididymis secretory protein Li 49 |
| UniProt Protein Name: | Triosephosphate isomerase |
| UniProt Synonym Protein Names: | Triose-phosphate isomerase |
| UniProt Gene Name: | TPI1 |
| UniProt Entry Name: | TPIS_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TPI1 antibody (CAB2579) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of HeLa cells using TPI1 antibody (CAB2579). Blue: DAPI for nuclear staining. |
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