Cell Biology Antibodies 14

Anti-TOP3A Antibody (CAB16987)

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SKU:
CAB16987
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TOP3A Antibody
Antibody SKU:CAB16987
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOP3A (XP_011522303.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse testis
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOP3A (XP_011522303.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MILA RNYL DVYP YDHW SDKI LPVY EQGS HFQP STVE MVDG ETSP PKLL TEAD LIAL MEKH GIGT DATH AEHI ETIK ARMY VGLT PDKR FLPG HLGM GLVE
Gene ID:7156
Uniprot:Q13472
Cellular Location:
Calculated MW:101kDa/109kDa/112kDa
Observed MW:112kDa
Anti-TOP3A Antibody (CAB16987)Western blot - TOP3A Rabbit pAb (CAB16987)
Synonyms:TOP3A, TOP3, ZGRF7
Background:This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.
NCBI Summary:This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
UniProt Code:Q13472
NCBI GenInfo Identifier:1002819406
NCBI Gene ID:7156
NCBI Accession:NP_001307688.1
UniProt Secondary Accession:Q13472,Q13473, A8KA61, B4DK80, D3DXC7,
UniProt Related Accession:Q13472
Molecular Weight:101,208 Da
NCBI Full Name:DNA topoisomerase 3-alpha isoform 2
NCBI Synonym Full Names:DNA topoisomerase III alpha
NCBI Official Symbol:TOP3A  
NCBI Official Synonym Symbols:TOP3; ZGRF7  
NCBI Protein Information:DNA topoisomerase 3-alpha
UniProt Protein Name:DNA topoisomerase 3-alpha
UniProt Synonym Protein Names:DNA topoisomerase III alpha
UniProt Gene Name:TOP3A  
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