Cell Biology Antibodies 14
Anti-TOP1 Antibody (CAB12409)
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- SKU:
- CAB12409
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TOP1 Antibody |
| Antibody SKU: | CAB12409 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human TOP1 |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse thymus, HeLa, MCF7, NIH/3T3, K-562 |
| Immunogen: | A synthesized peptide derived from human TOP1 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 7150 |
| Uniprot: | P11387 |
| Cellular Location: | Nucleus, nucleolus, nucleoplasm |
| Calculated MW: | 91kDa |
| Observed MW: | 100KDa |
 | Western blot - TOP1 Rabbit mAb (CAB12409) |
| Synonyms: | TOPI |
| Background: | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008] |
| UniProt Protein Function: | TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family. |
| UniProt Protein Details: | Protein type:EC 5.99.1.2; Isomerase; Nucleolus; Oncoprotein Chromosomal Location of Human Ortholog: 20q12 Cellular Component: nuclear chromosome; nucleolus; nucleoplasm; nucleus Molecular Function:chromatin binding; DNA binding; DNA topoisomerase type I activity; protein binding Biological Process: chromatin remodeling; chromosome segregation; circadian regulation of gene expression; circadian rhythm; DNA replication; DNA topological change; protein sumoylation; response to drug |
| NCBI Summary: | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P11387 |
| NCBI GenInfo Identifier: | 12644118 |
| NCBI Gene ID: | 7150 |
| NCBI Accession: | P11387.2 |
| UniProt Secondary Accession: | P11387,O43256, Q12855, Q12856, Q15610, Q5TFY3, Q9UJN0 A8KA78, E1P5W3, |
| UniProt Related Accession: | P11387 |
| Molecular Weight: | 90,726 Da |
| NCBI Full Name: | DNA topoisomerase 1 |
| NCBI Synonym Full Names: | topoisomerase (DNA) I |
| NCBI Official Symbol: | TOP1 |
| NCBI Official Synonym Symbols: | TOPI |
| NCBI Protein Information: | DNA topoisomerase 1 |
| UniProt Protein Name: | DNA topoisomerase 1 |
| UniProt Synonym Protein Names: | DNA topoisomerase I |
| Protein Family: | Crescerin |
| UniProt Gene Name: | TOP1 |
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