Cell Biology Antibodies 2

Cardiac troponin T (TNNT2) Rabbit Polyclonal Antibody (CAB1126)

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SKU:
CAB1126
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Mouse, Rat
Synonyms:
CMH2, RCM3, TnTC, cTnT, CMD1D, CMPD2, LVNC6, Cardiac troponin T (TNNT2)
Research Use:
Cell Biology
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Description

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Cardiac troponin T (TNNT2) Antibody (CAB1126)

The Cardiac troponin T (TNNT2) Antibody (CAB1126) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot applications, enabling accurate detection of TNNT2 in various cell types.TNNT2, also known as cardiac troponin T, is a key player in regulating muscle contraction in the heart.

This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.

Product Name:Cardiac troponin T (TNNT2) Antibody
SKU:CAB1126
Size:20μL, 100μL
Reactivity:Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSDI EEVV EEYE EEEQ EEAA VEEQ EEAA EEDA EAEA ETEE TRAE EDEE EEEA KEAE DGPM EESK PKPR SFMP NLVP PKIP DGER VDFD DIHR KRME KDLN ELQA LIEA HFEN RKKE EEEL
Tested Applications:WB IHC-P ELISA IF-P
Recommended Dilution:
WB1:1000 - 1:5000
IF-P1:50 - 1:200
IHC-P1:100 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CMH2, RCM3, TnTC, cTnT, CMD1D, CMPD2, LVNC6, Cardiac troponin T (TNNT2)
Positive Sample:Mouse heart, Rat heart
Cellular Localization:Cardiac Myofibril, Cytosol, Sarcomere, Striated Muscle Thin Filament.
Calculated MW:31-36kDa
Observed MW:40kDa

This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy.

Purification MethodAffinity purification
Gene ID7139
RRIDAB_2758480
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Cardiac troponin T (TNNT2) Rabbit pAb (CAB1126) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of paraffin-embedded rat heart using Cardiac troponin T (TNNT2) Rabbit pAb (CAB1126) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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