Cell Biology Antibodies 2
Anti-TNNT2 Antibody (CAB1126)
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- SKU:
- CAB1126
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TNNT2 Antibody |
| Antibody SKU: | CAB1126 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human TNNT2 (NP_001001431.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | mouse heart, rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human TNNT2 (NP_001001431.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSDI EEVV EEYE EEEQ EEAA VEEQ EEAA EEDA EAEA ETEE TRAE EDEE EEEA KEAE DGPM EESK PKPR SFMP NLVP PKIP DGER VDFD DIHR KRME KDLN ELQA LIEA HFEN RKKE EEEL |
| Gene ID: | 7139 |
| Uniprot: | P45379 |
| Cellular Location: | |
| Calculated MW: | 30kDa/31kDa/34kDa/35kDa |
| Observed MW: | 45kDa |
| Synonyms: | TNNT2, CMD1D, CMH2, CMPD2, LVNC6, RCM3, TnTC, cTnT, troponin T2 |
| Background: | The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. |
| UniProt Protein Function: | TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q32 Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament Molecular Function:troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; actin binding; tropomyosin binding Biological Process: atrial cardiac muscle morphogenesis; positive regulation of ATPase activity; metabolic process; regulation of muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2 |
| NCBI Summary: | The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq] |
| UniProt Code: | P45379 |
| NCBI GenInfo Identifier: | 48255877 |
| NCBI Gene ID: | 7139 |
| NCBI Accession: | NP_000355.2 |
| UniProt Secondary Accession: | P45379,O60214, Q99596, Q99597, Q9UM96, A2TDB9, A8K3K6 |
| UniProt Related Accession: | P45379,Q15607,Q15608,Q7Z554,Q8IZA1,Q9BUF6 |
| Molecular Weight: | 35,924 Da |
| NCBI Full Name: | troponin T, cardiac muscle isoform 1 |
| NCBI Synonym Full Names: | troponin T type 2 (cardiac) |
| NCBI Official Symbol: | TNNT2 |
| NCBI Official Synonym Symbols: | CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6; MGC3889 |
| NCBI Protein Information: | troponin T, cardiac muscle; OTTHUMP00000033864; OTTHUMP00000033865; OTTHUMP00000033866; OTTHUMP00000033867; OTTHUMP00000033870; OTTHUMP00000218095; troponin T2, cardiac; cardiac muscle troponin T; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant) |
| UniProt Protein Name: | Troponin T, cardiac muscle |
| UniProt Synonym Protein Names: | Cardiac muscle troponin T |
| Protein Family: | Troponin |
| UniProt Gene Name: | TNNT2 |
| UniProt Entry Name: | TNNT2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TNNT2 antibody (CAB1126) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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